Anti-Arp3 (C-terminal region) Antibody

Name: Anti-Arp3 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: AP4581
Price: 275.0 USD
Availability: InStock

3 Citations

Our Arp3 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Arp3 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

Immunocytochemical labeling of Arp3 in aldehyde-fixed and NP-40-permeabilized rat PC12 cells differentiated with NGF. The cells were labeled with rabbit polyclonal anti-Arp3 (C-terminal region) (AP4581) in the absence (left) or presence (right) of blocking peptide (AX4585). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot of human A431 (lane 1), Jurkat (lane 2), and HeLa (lane 3) cells. The blots were probed with rabbit polyclonal anti-Arp3 (C-terminal region) antibody at 1:1000 (lanes 1-3).

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

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from PhosphoSolutions

SKU: AP4581

Volume: 100 µL

Variant

Price:

$275

Quantity:

Product Details

Target

Arp3 (C-terminal region)

Target Description

Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. Several nucleation promoting factors, such as WASP and coronin, regulate the activity of the Arp2/3 complex. In addition, the Arp2/3 complex may be regulated by phosphorylation of specific subunits in the complex. Arp2 has two phosphosites, Thr-237 and Thr-238, that are evolutionarily conserved, and are phosphorylated along with Tyr-202 in response to growth factor stimulation. These phosphorylation events may regulate binding to the pointed end of actin filaments, and alanine substitutions of these Arp2 phosphosites inhibit membrane protrusions. Thus, phosphorylation may be another mode of Arp2/3 complex regulation in addition to the activity of nucleation-promoting factors.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

ACTR3

Molecular Weight

Antigen

Arp3 (C-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Arp3. This peptide sequence is highly conserved in rat, mouse, chicken, fish, and yeast Arp3 proteins.

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:250

Conjugate

Unconjugated

Specificity

The antibody detects a 47 kDa* doublet corresponding to the molecular mass of Arp3 on SDS-PAGE immunoblots of human A431, Jurkat, and HeLa cells, and in rat PC12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ACTR3, Arp3

UniProt Details

UniProt (Human): P61158
UniProt (Immunogen Species): P61158

Product Specific References for Applications and Species

GST Pulldown Assay: Mouse
Immunohistochemistry: Mouse
Western Blot: Mouse

GST pulldown assay: Mouse
PMID	Dilution	Publication
31063459	1:100	Galvan, D, et al. 2019. Drp1S600 phosphorylation regulates mitochondrial fission and progression of nephropathy in diabetic mice. Journal of clinical investigation, 2807-2823.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33388318	not listed	Zhao, Y, et al. 2021. mTORC1 and mTORC2 Converge on the Arp2/3 Complex to Promote KrasG12D-Induced Acinar-to-Ductal Metaplasia and Early Pancreatic Carcinogenesis. Gastroenterology, 1755-1770.
31063459	not listed	Galvan, D, et al. 2019. Drp1S600 phosphorylation regulates mitochondrial fission and progression of nephropathy in diabetic mice. Journal of clinical investigation, 2807-2823.

Western Blot: Mouse
PMID	Dilution	Publication
33388318	not listed	Zhao, Y, et al. 2021. mTORC1 and mTORC2 Converge on the Arp2/3 Complex to Promote KrasG12D-Induced Acinar-to-Ductal Metaplasia and Early Pancreatic Carcinogenesis. Gastroenterology, 1755-1770.

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Anti-Arp3 (C-terminal region) Antibody

Our Arp3 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, rat, and Xenopus Arp3 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

SKU: AP4581

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Arp3 (C-terminal region) Antibody

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