Our Anti-Aquaporin 2 (Ser264) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects rat Aquaporin 2 (Ser264) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Primary Antibody
Human, Mouse, Rat
Bovine, Canine, Chicken, Non-Human Primate
IHC, WB
Rabbit
Antigen Affinity Purified from Pooled Serum
AQP2
29/37 kDa
Product Specific References for Applications and Species
Immunohistochemistry: Mouse | ||
PMID | Dilution | Publication |
18606813 | 1:100 | Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. |
Immunohistochemistry: Rat | ||
PMID | Dilution | Publication |
26147297 | not listed | Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719. |
18606813 | 1:100 | Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. |
18287043 | not listed | Fenton, R.A., et al. 2007. Acute regulation of aquaporin-2 phosphorylation at serine-264 by vasopressin. Proc Natl Acad Sci U S A. 2008 Feb 26;105(8):3134-9. |
Western Blot: Human | ||
PMID | Dilution | Publication |
32529500 | not listed | Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8. |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
32529500 | not listed | Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8. |
29046300 | 1:3000 | Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391. |
18606813 | 1:2000 | Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. |
Western Blot: Rat | ||
PMID | Dilution | Publication |
29046300 | 1:3000 | Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391. |
27534994 | not listed | Al-Butaineh, M.M, et al. 2016. Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308. |
27185292 | not listed | Speed, J.S, et al. 2016. In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251. |
26147297 | not listed | Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719. |
25651562 | 1:1000 | Choi, H.J., et al. 2015. Extracellular pH affects phosphorylation and intracellular trafficking of AQP2 in inner medullary collecting duct cells. Am J Physiol Renal Physiol 308(7):F737-48. |
24259510 | 1:1000 | Hoffert, J.D., et al. 2014. Global analysis of the effects of the V2 receptor antagonist satavaptan on protein phosphorylation in collecting duct. American Journal of Physiology-Renal Physiology, 306(4), 410-421. |
18606813 | 1:2000 | Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27. |
16985212 | 1:2000 | Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700. |