Our Anti-5-Lipoxygenase (Ser523) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat 5-Lipoxygenase (Ser523) and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Human, Mouse, Rat
Non-Human Primate
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
ALOX5
80 kDa

Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
- Western Blot: Mouse
Western Blot: Mouse | ||
PMID | Dilution | Publication |
15280375 | not listed | Luo, M., et al. 2004. Luo, M., Jones, S.M., Phare, S.M., Coffey, M.J., Peters-Golden, M. and Brock, T.G., 2004. Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523. Journal of Biological Chemistry, 279(40), pp.41512-41520. |
Product Specific Protocols
- Western Blot Protocol: Download