Name: Anti-5-Lipoxygenase (Ser523) Antibody
Brand: PhosphoSolutions
SKU: p175-523
Price: 415.0 USD
Availability: InStock

Our Anti-5-Lipoxygenase (Ser523) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat 5-Lipoxygenase (Ser523) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

ALOX5

Molecular Weight

80 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~80 kDa doublet of 5-LO phosphorylated at Ser523 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-5-Lipoxygenase (Ser523) Antibody

Product Details

Target

5-Lipoxygenase (Ser523)

Target Description

The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

ALOX5

Molecular Weight

80 kDa

Antigen

Synthetic phosphopeptide corresponding to amino acid residues surrounding Ser523 of human 5-lipoxygenase (5-LO), conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2492028

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~80 kD 5-LO phosphorylated at Ser523 in Western blots. Immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-phosphatase, 1200 units for 30 min).

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser523

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

5 Lipoxygenase antibody, 5 LO antibody, 5 LOX antibody, 5-lipoxygenase antibody, 5-LO antibody, 5-LOX antibody, 5LOX antibody, 5LPG antibody, ALOX 5 antibody, Alox5 antibody, Arachidonate 5 lipoxygenase antibody, Arachidonate 5-lipoxygenase antibody, arachidonic 5-lipoxygenase alpha-10 isoform antibody, arachidonic 5-lipoxygenase delta-10-13 isoform antibody, arachidonic 5-lipoxygenase delta-13 isoform antibody, arachidonic 5-lipoxygenase delta-p10 isoform antibody, Arachidonic acid 5 lipoxygenase antibody, Leukotriene A4 synthase antibody, LOG 5 antibody, LOG5 antibody, LOX5_HUMAN antibody, MGC163204 antibody

UniProt Details

UniProt (Human): P09917
UniProt (Immunogen Species): P09917

NCBI Gene ID

240

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
15280375	not listed	Luo, M., et al. 2004. Luo, M., Jones, S.M., Phare, S.M., Coffey, M.J., Peters-Golden, M. and Brock, T.G., 2004. Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523. Journal of Biological Chemistry, 279(40), pp.41512-41520.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-5-Lipoxygenase (Ser523) Antibody

Our Anti-5-Lipoxygenase (Ser523) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat 5-Lipoxygenase (Ser523) and is antigen affinity purified from pooled serum. It is great for use in WB.