CamKII thr386 in human astrocytes

Anti-Phospho Thr286 Calcium/Calmodulin Kinase II (cat. p1005-286)

Our CamKII thr286 antibody works great in ICC.

CamKII in human astrocytes
Human astrocytes (1x106 cells/ml) were cultured for 24h without treatment or with METH (10 μM), clade B gp120 (50 ng/ml), or clade C gp120 (50 ng/ml) alone or METH in combination with clade B or C gp120. After incubation, the cells were fixed, permeabilized, and stained for phospho-CaMK II (green) or GFPA (red) and DAPI (blue). Image courtesy of Samikkannu T., et al. HIV Subtypes B and C gp120 and Methamphetamine Interaction: Dopaminergic System Implicates Differential Neuronal Toxicity. Sci. Rep. 2015;5:11130.

Involved in numerous signaling cascades, CamKII plays an important role in learning and memory.

​​Ca2+/calmodulin-dependent protein kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that transduces Ca2+ signals to several target proteins, including ion channels and transcription activators. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr-286 on the kinase produces an autonomously active form of CaM Kinase II which is required for various cellular functions including long-term potentiation (LTP) and spatial learning (Meng et al., 2003; Picciotto et al., 1993; Geis et al., 1998).

Western blots using our CamKII antibody show clean labeling of both the α and β subunits.

CamKII in rat brain
Western blot of rat brain lysate showing specific immunolabeling of the ~50 kDa α- and the ~60 kDa β-CaM Kinase II phosphorylated at Thr286 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Lots of researchers published with our CamKII thr286 antibody last year!

CamKII thr286 Publications, 2021
PMID Species Application Dilution Publication
33881939 Mouse WB 1:2000 Goodell, D.J., et al. 2021. Young DAPK1 knockout mice have altered presynaptic function.
33853773 Mouse WB 1:1000 Cook, S.G., et al. 2021. CaMKII holoenzyme mechanisms that govern the LTP versus LTD decision.
33449853 Mouse WB 1:1000 Kreitmeier, K.G., et al. 2021. CaMKIIδ Met281/282 oxidation is not required for recovery of calcium transients during acidosis.
34687697 Rat WB 1:1000 Rumian, N.L., et al. 2021. CaMKIIα knockout protects from ischemic neuronal cell death after resuscitation from cardiac arrest.
34544759 Rat WB 1:1000 Murray, C.H., et al. 2021. CaMKII modulates diacylglycerol lipase-α activity in the rat nucleus accumbens after incubation of cocaine craving.
33444640 Rat WB 1:1000 Murray, C.H., et al. 2021. mGlu5 function in the nucleus accumbens core during the incubation of methamphetamine craving.
  1. Gleason MR, Higashijima S, Dallman J, Liu K, Mandel G, Fetcho JR (2003). Nature Neurosci 6:217-218.
  2. Hudmon A, Schulman H (2002). Annu Rev Biochem 71:473-510.
  3. Meng FJ, Guo J, Zhang QG, Song B, Zhang GY (2003). Brain Res 967:161-169.
  4. Picciotto MR, Czernik AJ, Nairn AC (1993). J Biol Chem 268:26512-26521.
  5. Giese, KP, Fedorov, NB, Filipkowski, RK, Silva, AJ (1998). Science. 279(5352), 870–873
  6. Soderling TR (2000). Curr Opin Neurobiol 10:375-380.
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