Anti-VEGFR-3 (N-terminus) Antibody

Name: Anti-VEGFR-3 (N-terminus) Antibody
Brand: PhosphoSolutions
SKU: VP2941
Price: 255.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

FLT4

Molecular Weight

170

Go to Full Product Details

Left: Western blot image of human K-562 cells (lanes 1 & 2) and HUVEC (lanes 3 & 4). The blots were probed with rabbit polyclonal anti-VEGFR-3 (a.a. 1285-1298) in the absence (lanes 1 & 3) or presence of blocking peptide (VX2945) (lanes 2 & 4). Right: Western blot image of GST-recombinant human VEGFR-1 (89 kDa), VEGFR-2 (110 kDa), and VEGFR-3 (86 kDa) C-terminal regions. The blot was probed with anti-VEGFR-3 (a.a. 1285-1298).

Volume: 100 µL

Variant

Price:

$255

Quantity:

Bulk Order Anti-VEGFR-3 (N-terminus) Antibody

Product Details

Target

VEGFR-3 (N-terminus)

Target Description

Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

FLT4

Molecular Weight

170

Antigen

A synthetic peptide (coupled to carrier protein) corresponding to amino acids from the N-terminal region of human VEGFR-3. This sequence has two amino acid differences from rat VEGFR-3, and five amino acid differences from mouse VEGFR-3.

Species Reactivity

Human, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 170 kDa* band corresponding to VEGFR-3 in western blots of human endothelial and K-562 cells, and shows strong reactivity to recombinant human VEGFR-3, but not VEGFR-1 or VEGFR-2.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

FLT-4, Vascular endothelial growth factor receptor 3

UniProt Details

UniProt (Human): P35916
UniProt (Immunogen Species): P35916

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Anti-VEGFR-3 (N-terminus) Antibody