Anti-VEGFR-2 (Tyr-801) [conserved site], Phosphospecific Antibody

Name: Anti-VEGFR-2 (Tyr-801) [conserved site], Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: VP2921
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

4 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

KDR

Molecular Weight

180

Go to Full Product Details

Left: Western blot image of HUVEC cells untreated (-) or treated with pervanadate (1 mM) for 30 min. (+). Right: Western blot image of GST-recombinant VEGFR-2 kinase without (-) or with (+) akaline phosphatase treatment. Both sets of blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317) or anti-VEGFR-2 (Tyr-801).

Western blot image of GST-recombinant human VEGFR-1 (89 kDa), VEGFR-2 (110 kDa), and VEGFR-3 (86 kDa) C-terminal regions. The blots were probed with rabbit polyclonal anti-VEGFR-2 (a.a. 1304-1317), anti-VEGFR-2 (Tyr-801, conserved site), and anti-VEGFR-3 (a.a. 1285-1298).

Volume: 100 µL

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$309

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Bulk Order Anti-VEGFR-2 (Tyr-801) [conserved site], Phosphospecific Antibody

Product Details

Target

VEGFR-2 (Tyr-801)

Target Description

Vascular endothelial growth factor receptor-2 (VEGFR-2/Flk-1/KDR) is the primary receptor for VEGF in endothelial cells. Other VEGFR family members, VEGFR-1 (Flt-1) and VEGFR-3 (Flt-4), can also transduce the intracellular signals of VEGF. However, the role of VEGFR-1 is observed mainly during embryonic angiogenesis and VEGFR-3 signaling may be restricted to specific types of endothelial cells. Major autophosphorylation sites of VEGFR-2 are located in the kinase insert domain (Tyr-951/996) and in the tyrosine kinase catalytic domain (Tyr-1054/1059). Other sites, Tyr-1175 and Tyr-1212 provide docking sites for downstream signaling molecules. Activation of VEGFR-2 also phosphorylates Tyr-801, leading to PI3-kinase-Akt activation and increases in endothelial nitric oxide synthase activity. Phosphorylation of mutliple sites in VEGFR-2 is required for downstream activation of several signaling pathways that control proliferation, chemotaxis, and sprouting during angiogenesis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

KDR

Molecular Weight

180

Antigen

Phospho-VEGFR-2 (Tyr-801) peptide (coupled to carrier protein) corresponding to amino acids surrounding Tyr-801 in human VEGFR-2. This sequence has high homology to the conserved site in rat and mouse VEGFR-2, and has significant homology to the conserved sites in VEGFR-1 (Tyr-794) and VEGFR-3 (Tyr-812).

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 180 kDa* band corresponding to VEGFR-2 in western blots of human endothelial cells treated with pervanadate, and shows strong reactivity toward recombinant human VEGFR-2. This reactivity is removed by akaline phosphatase treatment. In addition, the antibody detects the conserved site in VEGFR-1 (Tyr-794). The antibody also works for immunofluorescent staining of VEGFR-2 in HUVEC cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr-801

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

KDR, flk-1, Vascular endothelial growth factor receptor 2

UniProt Details

UniProt (Human): P35968
UniProt (Immunogen Species): P35968

Product Specific References for Applications and Species

Western Blot: Mouse | Zebrafish

Western Blot: Mouse
PMID	Dilution	Publication
35551916	not listed	Mateluna, C, et al. 2022. Identification of VEGFR2 as the Histatin-1 Receptor in Endothelial Cells. Biochemical Pharmacology, 115079.
29955047	not listed	Baek, M, et al. 2018. Epidermal-specific deletion of TC-PTP promotes UVB-induced epidermal cell survival through the regulation of Flk-1/JNK signaling. Cell death & disease, 730.
24706887	not listed	Chen, PY, et al. 2014. The docking protein FRS2α is a critical regulator of VEGF receptors signaling. Proceedings of the National Academy of Sciences of the United States of America, 5514-5519.

Western Blot: Zebrafish
PMID	Dilution	Publication
24300896	not listed	Hartsough, EJ, et al. 2013. Lysine methylation promotes VEGFR-2 activation and angiogenesis. Science signaling, ra104.

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Anti-VEGFR-2 (Tyr-801) [conserved site], Phosphospecific Antibody