Our Anti-Tryptophan Hydroxylase (Ser58) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rabbit Tryptophan Hydroxylase (Ser58) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of recombinant tryptophan hydroxylase incubated in the absence (-) and presence (+) of cAMP-dependent protein kinase showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser58.
Anti-Tryptophan Hydroxylase (Ser58) Antibody
Tryptophan Hydroxylase (Ser58)
Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser-58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 of rat tryptophan hydroxylase 1 (TPH1), conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser58.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Kuhn, D.M, et al. 2007. Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II. Journal of Neurochemistry, 103(4):1567-73.
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