Name: Anti-Tryptophan Hydroxylase (Ser19) Antibody
Brand: PhosphoSolutions
SKU: p1575-19
Price: 415.0 USD
Availability: InStock

Our Anti-Tryptophan Hydroxylase (Ser19) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Tryptophan Hydroxylase (Ser19) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Rat

Expected Reactivity

Bovine, Zebrafish

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

TPH2

Molecular Weight

55 kDa

Go to Full Product Details

Western blot of recombinant tryptophan hydroxylase incubated in the absence (-) and presence (+) of Ca2+/calmodulin dependent kinase II showing specific immunolabeling of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser19.

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Tryptophan Hydroxylase (Ser19) Antibody

Product Details

Target

Tryptophan Hydroxylase (Ser19)

Target Description

Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996). CaM K II phosphorylates Ser-19 which lies within the regulatory domain of TPH2 (McKinney et al., 2005).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

TPH2

Molecular Weight

55 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser19 of rat tryptophan hydroxylase 2 (TPH2), conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Expected Reactivity

Bovine, Zebrafish

Antibody Registry ID

AB_2492272

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~55 kDa tryptophan hydroxylase protein phosphorylated at Ser19 .

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser19

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

MGC138871 antibody, ADHD7 antibody, FLJ37295 antibody, MGC138872 antibody, Neuronal tryptophan hydroxylase antibody, NTPH antibody, TPH 2 antibody, Tph2 antibody, TPH2 antibody, TPH2_HUMAN antibody, Tryptophan 5-hydroxylase 2 antibody, Tryptophan 5-monooxygenase 2 antibody, Tryptophan hydroxylase 2 antibody

UniProt Details

UniProt (Immunogen Species): Q8CGU9

NCBI Gene ID

317675

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
17727633	1:1000	Kuhn, D.M, et al. 2007. Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II. Journal of Neurochemisry, 103(4):1567-73.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Tryptophan Hydroxylase (Ser19) Antibody

Our Anti-Tryptophan Hydroxylase (Ser19) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Tryptophan Hydroxylase (Ser19) and is antigen affinity purified from pooled serum. It is great for use in WB.