Anti-TRPM8 (Extracellular region) Antibody

Name: Anti-TRPM8 (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: TP5701
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

3 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

TRPM8

Molecular Weight

130

Go to Full Product Details

Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with rabbit polyclonal anti-TRPM8 (TP5701). The antibody was detected using goat anti-rabbit DyLight® 594.

Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with rabbit polyclonal anti-TRPM8 (extracellular region) without peptide blocking (lane 1) or with peptide blocking (lane 2).

Volume: 100 µL

Variant

Price:

$309

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Product Details

Target

TRPM8 (Extracellular region)

Target Description

The Transient Receptor Potential Melastatin (TRPM) subfamily of cation-permeable channels is ubiquitous in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, some TRPM family members, TRPM6 and TRPM7, possess serine/threonine kinase activity and autophosphorylation. TRPM8 is thermoactivated at mildly cold temperatures (>25oC), and can also be activated by compounds that cause a cooling sensation, such as menthol and icilin. TRPM8 is expressed in trigeminal and dorsal root ganglia neurons where it confers sensitivity to cold in the somatosensory system. In vascular smooth muscle, TRPM8 may alter blood flow by constricting or enlarging blood vessels. TRPM8 is also expressed in normal prostate epithelial cells, as well as overexpressed in several primary tumors including colon, lung, skin, breast, and prostate cancers.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

TRPM8

Molecular Weight

130

Antigen

TRPM8 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM8. This site is well conserved in rat and mouse TRPM8, but has low homology to other TRPM family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
ICC: 1:200

Conjugate

Unconjugated

Specificity

This antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 and MCF-7 cells. The antibody is also recommended for immunocytochemical labeling of cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

LTrpC6, TRPp8, TRP, TRPM8,

UniProt Details

UniProt (Human): Q7Z2W7
UniProt (Immunogen Species): Q7Z2W7

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Mouse | Sheep

Immunocytochemistry: Mouse
PMID	Dilution	Publication
36555804	1:200	Hernández-Ortego, P, et al. 2022. Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression. International journal of molecular sciences, 16164.

Western Blot: Mouse
PMID	Dilution	Publication
36555804	1:500	Hernández-Ortego, P, et al. 2022. Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression. International journal of molecular sciences, 16164.

Western Blot: Sheep
PMID	Dilution	Publication
37482368	1:500	Sutton, CM, et al. 2023. Expression of TRPM8 in Sheep Reproductive Tissues and Brain Areas Important for the Expression of Reproductive Behavior. Physiology & Behavior, 114304.

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Anti-TRPM8 (Extracellular region) Antibody