Anti-TRPM8 (Extracellular region) Antibody (M571)

Name: Anti-TRPM8 (Extracellular region) Antibody
Brand: PhosphoSolutions
SKU: TM5711
Price: 360.0 USD
Availability: InStock

1 Citation

Our TRPM8 (Extracellular region) mouse monoclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat TRPM8 (Extracellular region) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of TRPM8 in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-TRPM8 (M571). The antibody was detected using goat anti-mouse DyLight® 594.

Western blot image of human TRPM8 in human MDA-MB-231 cells. The blot was probed with mouse monoclonal anti-TRPM8 (extracellular region) clone M571 (lane 1) or clone M572 (lane 2).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

Go to Full Product Details

SKU: TM5711

Product Details

Target

TRPM8 (Extracellular region)

Target Description

The Transient Receptor Potential Melastatin (TRPM) subfamily of cation-permeable channels is ubiquitous in mammalian tissues. This family includes TRPM1-8. In addition to acting as a calcium-permeant channel, some TRPM family members, TRPM6 and TRPM7, possess serine/threonine kinase activity and autophosphorylation. TRPM8 is thermoactivated at mildly cold temperatures (>25oC), and can also be activated by compounds that cause a cooling sensation, such as menthol and icilin. TRPM8 is expressed in trigeminal and dorsal root ganglia neurons where it confers sensitivity to cold in the somatosensory system. In vascular smooth muscle, TRPM8 may alter blood flow by constricting or enlarging blood vessels. TRPM8 is also expressed in normal prostate epithelial cells, as well as overexpressed in several primary tumors including colon, lung, skin, breast, and prostate cancers.

Format

Antigen Affinity Purified

Clonality

Monoclonal

Clone

M571

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

TRPM8

Molecular Weight

130

Antigen

Clone M571 was generated from TRPM8 synthetic peptide (coupled to carrier) corresponding to amino acids in the extracellular region of human TRPM8. This site is well conserved in rat and mouse TRPM8, but has low homology to other TRPM family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

This antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 and MCF-7 cells. The antibody is also recommended for immunocytochemical labeling of cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

LTrpC6, TRPp8, TRP, TRPM8,

UniProt Details

UniProt (Human): Q7Z2W7
UniProt (Immunogen Species): Q7Z2W7

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
36555804	1:200	Hernández-Ortego, P, et al. 2022. Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression. International journal of molecular sciences, 16164.

Western Blot: Mouse
PMID	Dilution	Publication
36555804	1:500	Hernández-Ortego, P, et al. 2022. Validation of Six Commercial Antibodies for the Detection of Heterologous and Endogenous TRPM8 Ion Channel Expression. International journal of molecular sciences, 16164.

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Anti-TRPM8 (Extracellular region) Antibody (M571)

SKU: TM5711

Product Details

Product Specific References for Applications and Species

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