Our Anti-Troponin I (cardiac) Ser150 rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Troponin I (cardiac) Ser150 and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of mouse heart lysate showing specific immunolabeling of ~25 kDa cardiac troponin I protein phosphorylated at Ser150 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Anti-Troponin I (Cardiac) Ser150 Antibody
Bulk Order Anti-Troponin I (Cardiac) Ser150 Antibody
Troponin I (cardiac) Ser150
Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser-23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser-23 and Ser-150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser-150 over Ser-23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).
Antigen Affinity Purified from Pooled Serum
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with λ-phosphatase treatment.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
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