Name: Anti-Troponin I (Cardiac) Ser150 Antibody
Brand: PhosphoSolutions
SKU: p2010-150
Price: 415.0 USD
Availability: InStock

Our Anti-Troponin I (cardiac) Ser150 rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Troponin I (cardiac) Ser150 and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Non-Human Primate

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

TNNI3

Molecular Weight

25 kDa

Go to Full Product Details

Western blot of mouse heart lysate showing specific immunolabeling of ~25 kDa cardiac troponin I protein phosphorylated at Ser150 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Troponin I (Cardiac) Ser150 Antibody

Product Details

Target

Troponin I (cardiac) Ser150

Target Description

Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser-23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser-23 and Ser-150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser-150 over Ser-23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

TNNI3

Molecular Weight

25 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 of mouse troponin I, cardiac (cTnI), conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2492270

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~25 kDa cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with λ-phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser150

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

cardiac muscle antibody, Cardiac troponin I antibody, Cardiac Troponin I antibody, cardiomyopathy dilated 2A (autosomal recessive) antibody, Cardiomyopathy familial hypertrophic 7 included antibody, CMD1FF antibody, CMD2A antibody, CMH7 antibody, cTnI antibody, Familial hypertrophic cardiomyopathy 7 antibody, MGC116817 antibody, RCM1 antibody, Tn1 antibody, Tni antibody, TNN I3 antibody, TNNC 1 antibody, TNNC1 antibody, TNNI3 antibody, TNNI3_HUMAN antibody, Troponin I antibody, Troponin I cardiac antibody, Troponin I cardiac muscle antibody, Troponin I cardiac muscle isoform antibody, Troponin I type 3 cardiac antibody, troponin I cardiac 3 antibody, TroponinI antibody, Troponin I type 3 (cardiac) antibody

UniProt Details

UniProt (Human): P19429
UniProt (Immunogen Species): P48787

NCBI Gene ID

21954

Product Specific References for Applications and Species

Western Blot: Human | Mouse

Western Blot: Human
PMID	Dilution	Publication
24291343	1:1000	Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71.
23564307	1:1000	Walker, L.A., et al. 2013. Contractile protein phosphorylation predicts human heart disease phenotypes. American Journal of Physiology-Heart and Circulatory Physiology, 304(12), pp.H1644-H1650.

Western Blot: Mouse
PMID	Dilution	Publication
24291343	1:1000	Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Troponin I (Cardiac) Ser150 Antibody

Our Anti-Troponin I (cardiac) Ser150 rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Troponin I (cardiac) Ser150 and is antigen affinity purified from pooled serum. It is great for use in WB.