Our Anti-Troponin I (cardiac) Ser150 rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Troponin I (cardiac) Ser150 and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Mouse, Rat
Non-Human Primate
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
TNNI3
25 kDa

Western blot of mouse heart lysate showing specific immunolabeling of ~25 kDa cardiac troponin I protein phosphorylated at Ser150 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely greatly decreased by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
Product Specific References for Applications and Species
Western Blot: Human | ||
PMID | Dilution | Publication |
24291343 | 1:1000 | Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71. |
23564307 | 1:1000 | Walker, L.A., et al. 2013. Contractile protein phosphorylation predicts human heart disease phenotypes. American Journal of Physiology-Heart and Circulatory Physiology, 304(12), pp.H1644-H1650. |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
24291343 | 1:1000 | Thoemmes, S.F., et al. 2014. Characterization and validation of new tools for measuring site-specific cardiac troponin I phosphorylation. Journal of Immunological Methods, 403(1-2), pp.66-71. |
Product Specific Protocols
- Western Blot Protocol: Download