Anti-TFAM (Transcription Factor A, Mitochondrial) Antibody
Our Anti-TFAM (Transcription Factor A, mitochondrial) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat TFAM (Transcription Factor A, motochondrial) and is neat serum. It is great for use in WB.
SKU: 2001-TFAM
Product Details
TFAM (Transcription Factor A, motochondrial)
Mitochondrial Transcription Factor A (TFAM) is a key activator of mitochondrial (mt) DNA transcription as well as a participant in mitochondrial genome replication. mtDNA is highly susceptible to oxidative stress leading to mitochondrial dysfunction. Overexpression of TFAM has been implicated in the amelioration of age dependent impairment of brain functions through the prevention of oxidative stress and mitochondrial dysfunction in microglia (Hayashi et al., 2008). More recently, TFAM overexpression has been shown to potentially reduce oxidative stress in motor neurons and delay onset of amyotrophic lateral sclerosis (ALS) in ALS model mice (Morimoto et al., 2012).
Neat Serum
Polyclonal
IgG
WB
Rabbit
TFAM
24 kDa
Native recombinant mouse TFAM protein with c-terminal 6-his tag
Human, Mouse, Rat
AB_2492259
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Neat Serum
Neat Serum
WB: 1:2000
Unconjugated
Specific for endogenous levels of the ~24 kDa TFAM protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Transcription factor 6-like 1 antibody, Mitochondrial transcription factor 1 antibody, mitochondrial transcription factor A antibody, MtTF1 antibody, mtTFA antibody, TCF 6 antibody, TCF-6 antibody, TCF6 antibody, TCF6L1 antibody, TCF6L2 antibody, TCF6L3 antibody, TFAM antibody, TFAM_HUMAN antibody, Transcription factor 6 antibody, Transcription factor 6 like 2 (mitochondrial transcription factor) antibody, Transcription factor 6 like 2 antibody, Transcription factor 6-like 2 antibody, transcription factor 6-like 3 antibody, Transcription factor A mitochondrial antibody, Transcription factor A mitochondrial antibody, Transcription factor A mitochondrial precursor antibody
UniProt (Immunogen Species): P40630
21780
Product Specific References for Applications and Species
- Western Blot: Mouse
Western Blot: Mouse | ||
PMID | Dilution | Publication |
37298383 | not listed | Kozhukhar, N, et al. 2023. The C-Terminal Tail of Mitochondrial Transcription Factor A Is Dispensable for Mitochondrial DNA Replication and Transcription In Situ. International Journal of Molecular Sciences, 9430. |
35883613 | not listed | Kozhukhar, N., et al. 2022. A Method for In Situ Reverse Genetic Analysis of Proteins Involved mtDNA Replication. Cells, 2168. |
35487893 | 1:1000 | Sidarala, V., et al. 2022. Mitofusin 1 and 2 regulation of mitochondrial DNA content is a critical determinant of glucose homeostasis. Nature Communications, 13(1), pp.1-16. |
Product Specific Protocols
- Western Blot Protocol: Download
General References
- Hayashi, Y., Yoshida, M., Yamato, M., Ide, T., Wu, Z., Ochi-Shindou, M., Kanki, T., Kang, D., Sunagawa, K., Tsutsui, H. and Nakanishi, H., 2008. Reverse of age-dependent memory impairment and mitochondrial DNA damage in microglia by an overexpression of human mitochondrial transcription factor a in mice. Journal of Neuroscience, 28(34), pp.8624-8634. PMID: 18716221
- Morimoto, N., Miyazaki, K., Kurata, T., Ikeda, Y., Matsuura, T., Kang, D., Ide, T. and Abe, K., 2012. Effect of mitochondrial transcription factor a overexpression on motor neurons in amyotrophic lateral sclerosis model mice. Journal of Neuroscience Research, 90(6), pp.1200-1208. PMID: 22354563