Anti-Synaptophysin 2 Antibody

Name: Anti-Synaptophysin 2 Antibody
Brand: PhosphoSolutions
SKU: 1950-SNT2
Price: 430.0 USD
Availability: InStock

1 Citation

Our Anti-Synaptophysin 2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Synaptophysin 2 and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Immunostaining of 40DIV cultured rat cortical neurons showing punctate labeling of synaptophysin (cat. 1950-SNT2, red, 1:100). The blue is staining nuclear DNA. Cells and photo courtesy of QBMCellScience.

Western blot of rat hippocampal lysate showing specific labeling of the ~38 kDa Synaptophysin2 protein.

Species Reactivity

Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: 1950-SNT2

Volume: 100 µL

Variant

Price:

$430

Quantity:

Product Details

Target

Synaptophysin 2

Target Description

Synaptophysin 2, also known as Synaptoporin, is a component of the synaptic vesicle membrane and thought to play an important role in synaptic vesicle trafficking (Dai et al., 2003). It is highly homologous to Synaptophysin 1, yet differs in expression. Whereas Synaptophysin 1 is ubiquitously expressed, Synaptophysin 2 is concentrated in the mossy fiber synapses of the hippocampus (Grabs et al., 1994; Singec et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

SYNPR

Molecular Weight

38 kDa

Antigen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat synaptophysin 2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Antibody Registry ID

AB_2492250

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~38 kDa Synaptophysin 2 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DKFZp686G0883 antibody, MGC26651 antibody, SPO antibody, Synaptophysin 2 antibody, Synaptoporin antibody, Synpr antibody, SYNPR_HUMAN antibody

UniProt Details

UniProt (Immunogen Species): P22831

NCBI Gene ID

66030

Product Specific References for Applications and Species

Immunohistochemistry: Mouse

Immunohistochemistry: Mouse
PMID	Dilution	Publication
26892644	not listed	Kraft, A.D., et al. 2016. Association Between Microglia, Inflammatory Factors, and Complement with Loss of Hippocampal Mossy Fiber Synapses Induced by Trimethyltin. Neurotoxicity Research, 1-14.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Synaptophysin 2 Antibody

SKU: 1950-SNT2

Product Details

Product Specific References for Applications and Species

Product Specific Protocols

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