Name: Anti-Synapsin I (Ser9) Antibody
Brand: PhosphoSolutions
SKU: p1560-9
Price: 415.0 USD
Availability: InStock

Our Anti-Synapsin I (Ser9) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse, rat, and zebrafish Synapsin I (Ser9) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Human, Xenopus

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SYN1

Molecular Weight

78 kDa

Go to Full Product Details

Western blot of rat cortical lysate showing specific immunolabeling of the ~78 kDa synapsin I phosphorylated at Ser9 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Immunostaining of cultured mouse caudate neurons showing synapsin I when phosphorylated at Ser9 (cat. p1560-9, 1:500, red). Cells and photo courtesy of QBMCellScience.

Volume: 100 µL

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$415

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Product Details

Target

Synapsin I (Ser9)

Target Description

Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogenesis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Serine 9 is the site on synapsin I that is phosphorylated by cAMP-dependent protein kinase and by calcium calmodulin kinase I (Czernik et al., 1987). Phosphorylation of this site is thought to regulate synaptic vesicle function and neurite outgrowth (Kao et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

SYN1

Molecular Weight

78 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Human, Xenopus

Antibody Registry ID

AB_2492244

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~78 kDa synapsin I doublet protein phosphorylated at Ser9. Also weakly labels the ~55 kDa synapsin II protein which has a similar phosphorylation site to that of Ser9 on synapsin I. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser9

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody

UniProt Details

UniProt (Human): P17600
UniProt (Immunogen Species): P17599

NCBI Gene ID

281510

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Mouse | Zebrafish

Immunocytochemistry: Mouse
PMID	Dilution	Publication
25609616	1:200	Shulman, Y., et al. 2015. ATP binding to synaspsin IIa regulates usage and clustering of vesicles in terminals of hippocampal neurons. Journal of Neuroscience, 35(3), pp.985-998.

Western Blot: Mouse
PMID	Dilution	Publication
26514267	1:1000	Benleulmi-Chaachoua, A., et al. 2016. Protein interactome mining defines melatonin MT 1 receptors as integral component of presynaptic protein complexes of neurons. Journal of Pineal Research, 60(1), pp.95-108.

Western Blot: Zebrafish
PMID	Dilution	Publication
20097902	not listed	Shimomura, S., et al. 2010. Identification of an endogenous substrate of zebrafish doublecortin-like protein kinase using a highly active truncation mutant. The Journal of Biochemistry, 147(5), pp.711-722.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Synapsin I (Ser9) Antibody

Our Anti-Synapsin I (Ser9) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse, rat, and zebrafish Synapsin I (Ser9) and is antigen affinity purified from pooled serum. It is great for use in WB, ICC.