Anti-Stat1 Antibody (M249)

Name: Anti-Stat1 Antibody
Brand: PhosphoSolutions
SKU: SM2491
Price: 340.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M249

Applications

ICC, IP, WB

Host Species

Mouse

Format

Protein A Purified

Gene Name

STAT1

Molecular Weight

84/91

Go to Full Product Details

Immunocytochemical labeling of Stat1 in human A431 cells. The cells were labeled with mouse monoclonal Stat1 (SM2491) antibody (Right), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. Corresponding phase image is shown to the left.

Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7 & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8 & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).

Volume: 100 µL

Variant

Price:

$340

Quantity:

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Product Details

Target

Stat1

Target Description

The stat proteins function both as cytoplasmic signal transducers and as activators of transcription. Stat1 is expressed as two variants of 84 and 91 kDa. Stat1 proteins contain SH2 and SH3 domains, and are components of the interferon-stimulated gene factor 3 (ISGF3) complex. This complex is the primary transcription activator induced by the binding of interferon to its receptors. In response to activation by various cytokines and growth factors, stat1 subunits become phosphorylated at tyrosine 701. This leads to translocation of stat1 to the nucleus, resulting in formation of an active ISGF3 complex. Active ISGF3 modulates the transcription of the interferon-stimulated genes. Thus, phosphorylation of Tyr-701 is critical for gene expression mediated by various cytokines and growth factors.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M249

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

STAT1

Molecular Weight

84/91

Antigen

Clone (M249) was generated from a recombinant protein from human Stat1. This sequence has high homology to the conserved region in rat and mouse stat1 a and b.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects 84 and 91 kDa* stat1 variants on SDS-PAGE immunoblots of human Jurkat and A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Stat1a, Stat1b, ISGF3, Stat1alpha, Stat1beta

UniProt Details

UniProt (Human): P42224
UniProt (Immunogen Species): P42224

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Stat1 Antibody (M249)

Anti-Stat1 Antibody
SKU: SM2491

Bulk Order Anti-Stat1 Antibody

Product Details

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