Anti-Sox2 (Thr-118), Phosphospecific Antibody

Name: Anti-Sox2 (Thr-118), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: SP5521
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

2 Citations

Product Type

Primary Antibody

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

SOX2

Molecular Weight

Go to Full Product Details

Immunocytochemical labeling of phosphorylated Sox2 in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with rabbit polyclonal anti-Sox2 (Thr-118)
phospho-specific (SP5521). The antibody was detected using goat anti-rabbit DyLight® 594.

Western blot image of mouse F9 stem cells treated with with calyculin A (100 nM, 30 min.) (lanes 1-4) then Sox2 was dephosphorylated with lambda phosphatase (lanes 2 & 4). The blot was probed with mouse monoclonal Sox2 (lanes 1 & 2) and rabbit polyclonal anti-Sox2 (Thr-118) phospho-specific antibody (lanes 3 & 4).

Volume: 100 µL

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$309

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Product Details

Target

Sox2 (Thr-118)

Target Description

Embryonic stem cells can maintain a pluripotent state that is controlled by a set of transcription factors that include Oct-4, Sox2, and Nanog. Chromatin immunoprecipitation experiments show that Sox2 and Oct-4 bind to thousands of gene regulatory sites, many of which regulate cell pluripotency and early embryonic development. siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency, while overexpression of Oct-4 and Sox2, along with additional transcription factors Klf4 and c-Myc, can reprogram somatic cells to a pluripotent state. Sox2 also regulates adult multipotent progenitors in various epithelial tissues, and may be important for survival and regeneration of these tissues. The activity of Sox2 may be regulated by phosphorylation and methylation. Akt1 phosphorylates Thr-118 and enhances Sox2 transcriptional activity, while Set7 can monomethylate Lys-119 leading to inhibition of Sox2 transcriptional activity, as well as Sox2 ubiquination and degradation. In addition, Sox2 Thr-128 is constituitively phoshorylated in the F9 mouse stem cell line.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

SOX2

Molecular Weight

Antigen

Phospho-Sox2 (Thr-118) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Thr-118 in mouse Sox2. This site is well conserved in rat and human Sox2, and has high homology with the conserved site in Sox1 (Thr-126) and Sox3 (Thr-144), but low homology to other Sox family members.

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody was cross adsorbed to unphosphorylated Sox2 (Thr-118) peptide before affinity purification using phospho-Sox2 (Thr-118) peptide. This antibody antibody detects a 34 kDa* protein on SDS-PAGE immunoblots of mouse F9 stem cells. This reactivity is not observed after lambda phosphatase dephosphorylation of Sox2.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr-118

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SRY (sex determining region Y) box-2, ANOP3, MCOPS3, SOX-2

UniProt Details

UniProt (Human): P48431
UniProt (Immunogen Species): P48432

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Immunohistochemistry: Mouse

Immunocytochemistry: Mouse
PMID	Dilution	Publication
28607354	1:200	Mir, S, et al. 2017. IGF-1 mediated Neurogenesis Involves a Novel RIT1/Akt/Sox2 Cascade. Scientific reports, 3283.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28007959	not listed	Mir, S, et al. 2017. RIT1 GTPase Regulates Sox2 Transcriptional Activity and Hippocampal Neurogenesis. The Journal of biological chemistry, 2054-2064.

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Anti-Sox2 (Thr-118), Phosphospecific Antibody