Western blot analysis of SMAD7 in human HepG2 cells. The blot was probed with rabbit polyclonal SMAD7 antibody (SP4901) at 1:250.
Anti-SMAD7 (N-terminal region) Antibody
Bulk Order Anti-SMAD7 (N-terminal region) Antibody
SMAD7 (N-terminal region)
SMADs are members of the MAD-related family of molecules. MAD-related proteins are a family of intracellular proteins that are essential components in the signaling pathways of the serine/threonine kinase receptors of the transforming growth factor beta superfamily. SMADs can be divided into receptor-regulated SMADs (R-SMADs: SMAD1, 2, 3. 5 and 8), common-mediator SMAD (co-SMAD: SMAD4), and inhibitory SMADs (I-SMADs: SMAD6 and 7). Briefly, activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved SSXS motif at the carboxy-terminus of the proteins. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad, Smad4, and together the complex moves to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.
Protein G Purified
This antibody was raised against amino acids from the N-terminal region of human SMAD7. This sequence is 100% or highly conserved between human and multiple species.
Frog, Human, Mouse, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
PBS + 0.05% BSA and 0.05% NaN3
This antibody detects SMAD7 at 46 kDa* on SDS-PAGE immunoblots of human Jurkat cells and mouse C2C12. The antibody can used for western blot and immunohistochemistry.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.