Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).
Anti-Slingshot-1L (C-terminal region) Antibody
SKU: SP1711
Bulk Order Anti-Slingshot-1L (C-terminal region) Antibody
Product Details
Slingshot-1L (C-terminal region)
Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.
Antigen Affinity Purified
Polyclonal
IgG
ICC, IHC, WB
Rabbit
SSH1
150
A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human SSH1L. This sequence has 100% homology with similar regions of mouse and bovine SSH1L, and has low homology to SSH2L and SSH3L.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
IHC: 1:100
ICC: 1:250
Unconjugated
The antibody detects a 150 kDa* protein corresponding to the molecular mass of SSH1L on SDS-PAGE immunoblots of PC12 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
