Anti-Slingshot-1L (C-terminal region) Antibody

Name: Anti-Slingshot-1L (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SP1711
Price: 329.0 USD
Availability: InStock

26 Citations

Our Slingshot-1L (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Slingshot-1L (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC, IHC.

Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).

Formalin fixed, citric acid treated parafin sections of mouse cerebral cortex. Sections were probed with anti-Slingshot-1L (SP1711) then anti-Rabbit:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).

DU-145 cells were transfected with control (Ctrl siRNAs) or AKAP2 specific siRNAs (siRNA1 and siRNA2). 72 h after transfection, cell lysates were loaded on SDS-PAGE gels and analyzed by Western blot. Phospho-SSH1 was detected using antibodies recognizing phospho-serines 937 and 978 (cat. SP3901). The amounts of SSH1 (cat. SP1711) and AKAP2 were detected using specific antibodies, as indicated. Image from publication CC-BY-4.0. PMID: 37827203

Western blot of human recombinant SSH1L untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) and rat PC12 cells unstimulated (lanes 5 & 7) or stimulated with calyculin A (lanes 6 & 8). The blots were probed with anti-SSH1L (C-term.) (lanes 1, 2, 5, & 6) or anti-SSH1L (Ser-978) (lanes 3, 4, 7, & 8).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Rabbit

Go to Full Product Details

SKU: SP1711

Product Details

Target

Slingshot-1L (C-terminal region)

Target Description

Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cell motility. Regulation of cofilin activity can occur through serine phosphorylation and dephosphorylation. Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. Multiple phosphatases, Slingshot, PP1, PP2A, PP2B, and chronophin can dephosphorylate Ser-3 and activate actin binding. In mammals, the Slingshot family includes SSH1L, SSH2L, and SSH3L. SSH1L and SSH2L mRNAs are widely expressed, while SSH3L has high expression in epithelial tissues. SSH1L can associate with F-actin and may be the major phosphatase regulating cofilin activity. Disruption of SSH1L expression using RNA interference impairs directional cell migration. Thus, Slingshot phosphatases may be critical for regulating cytoskeletal protein activity and cell motility.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, WB

Host Species

Rabbit

Gene Name

SSH1

Molecular Weight

150

Antigen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human SSH1L. This sequence has 100% homology with similar regions of mouse and bovine SSH1L, and has low homology to SSH2L and SSH3L.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
IHC: 1:100
ICC: 1:250

Conjugate

Unconjugated

Specificity

The antibody detects a 150 kDa* protein corresponding to the molecular mass of SSH1L on SDS-PAGE immunoblots of PC12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SSH1L

UniProt Details

UniProt (Human): Q8WYL5
UniProt (Immunogen Species): Q8WYL5

Product Specific References for Applications and Species

Immunocytochemistry: Human | Mouse
Immunohistochemistry: Human | Mouse
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Human
PMID	Dilution	Publication
25698445	not listed	Woo, JA, et al. 2015. Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via Aβ ligation to β1-integrin conformers. Cell death and differentiation, 921-934.

Immunocytochemistry: Mouse
PMID	Dilution	Publication
37463212	not listed	Cazzaro, S, et al. 2023. Slingshot Homolog-1-mediated Nrf2 Sequestration Tips the Balance from Neuroprotection to Neurodegeneration in Alzheimer's Disease. PNAS: USA, e2217128120.
36637427	1:100	Cazzaro,S, et al. 2023. Slingshot homolog-1 amplifies mitochondrial abnormalities by distinctly impairing health and clearance of mitochondria. Human molecular genetics, 1660-1672.
34342389	not listed	Lee, KY, et al. 2021. Infantile spasms-linked Nedd4-2 mediates hippocampal plasticity and learning via cofilin signaling. EMBO reports, e52645.
33044112	not listed	Fang, C, et al. 2021. SSH1 impedes SQSTM1/p62 flux and MAPT/Tau clearance independent of CFL (cofilin) activation. Autophagy, 2144-2165.
31577955	not listed	Zhang, Z, et al. 2019. The Schizophrenia Susceptibility Gene OPCML Regulates Spine Maturation and Cognitive Behaviors through Eph-Cofilin Signaling. Cell reports, 49-61.
25698445	not listed	Woo, JA, et al. 2015. Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via Aβ ligation to β1-integrin conformers. Cell death and differentiation, 921-934.

Immunohistochemistry: Human
PMID	Dilution	Publication
34284854	not listed	Li, M, et al. 2021. Phosphorylation of Cofilin-1 Enhances Paclitaxel Resistance of Epithelial Ovarian Cancer Cells by Inhibiting Apoptosis. Biomedical and environmental sciences : BES, 465-477.
25698445	not listed	Woo, JA, et al. 2015. Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via Aβ ligation to β1-integrin conformers. Cell death and differentiation, 921-934.
25684665	1:100	Wang, Y, et al. 2015. Cofilin-phosphatase slingshot-1L (SSH1L) is over-expressed in pancreatic cancer (PC) and contributes to tumor cell migration. Cancer Letters, 171-176.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36637427	1:100	Cazzaro,S, et al. 2023. Slingshot homolog-1 amplifies mitochondrial abnormalities by distinctly impairing health and clearance of mitochondria. Human molecular genetics, 1660-1672.
25698445	not listed	Woo, JA, et al. 2015. Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via Aβ ligation to β1-integrin conformers. Cell death and differentiation, 921-934.

Western Blot: Human
PMID	Dilution	Publication
37827203	not listed	Reggi, E, et al. 2023. AKAP2-anchored protein phosphatase 1 controls prostatic neuroendocrine carcinoma cell migration and invasion. Biochimica et biophysica acta. Molecular basis of disease, 166916.
25698445	not listed	Woo, JA, et al. 2015. Slingshot-Cofilin activation mediates mitochondrial and synaptic dysfunction via Aβ ligation to β1-integrin conformers. Cell death and differentiation, 921-934.
19339277	not listed	Kim, JS, et al. 2009. Reactive oxygen species regulate a slingshot-cofilin activation pathway. Moleculare biology of the cell, 2650-2660.

Western Blot: Mouse
PMID	Dilution	Publication
37463212	not listed	Cazzaro, S, et al. 2023. Slingshot Homolog-1-mediated Nrf2 Sequestration Tips the Balance from Neuroprotection to Neurodegeneration in Alzheimer's Disease. PNAS: USA, e2217128120.
36637427	1:1000	Cazzaro,S, et al. 2023. Slingshot homolog-1 amplifies mitochondrial abnormalities by distinctly impairing health and clearance of mitochondria. Human molecular genetics, 1660-1672.
34342389	not listed	Lee, KY, et al. 2021. Infantile spasms-linked Nedd4-2 mediates hippocampal plasticity and learning via cofilin signaling. EMBO reports, e52645.
33044112	not listed	Fang, C, et al. 2021. SSH1 impedes SQSTM1/p62 flux and MAPT/Tau clearance independent of CFL (cofilin) activation. Autophagy, 2144-2165.
31577955	not listed	Zhang, Z, et al. 2019. The Schizophrenia Susceptibility Gene OPCML Regulates Spine Maturation and Cognitive Behaviors through Eph-Cofilin Signaling. Cell reports, 49-61.
31400829	1:500	Tedeschi, A, et al. 2019. ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNS. Neuron, 1073-1085.
24464040	1:1000	Parisiadou, L, et al. 2014. LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity. Nature neuroscience, 367-376.

Western Blot: Rat
PMID	Dilution	Publication
37138671	1:1000	Ramos-Alvarez, I, et al. 2023. Cofilin Activation in Pancreatic Acinar Cells Plays a Pivotal Convergent Role for Mediating CCK-stimulated Enzyme Secretion and Growth. Frontiers in Physiology, 1147572.
28923249	1:500	Caso, S, et al. 2017. AKAP-Lbc mediates protection against doxorubicin-induced cardiomyocyte toxicity. Biochimica et biophysica acta. Molecular cell research, 2336-2346.
27073215	1:375	Lauterborn, J, et al. 2017. Cofilin Activation Is Temporally Associated with the Cessation of Growth in the Developing Hippocampus. Cerebral cortex (New York, N.Y. : 1991), 2640-2651.
24962708	1:800	Li, Y, et al. 2014. LIMK-dependent actin polymerization in primary sensory neurons promotes the development of inflammatory heat hyperalgesia in rats. Science signaling, ra61.

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Anti-Slingshot-1L (C-terminal region) Antibody

SKU: SP1711

Product Details

Product Specific References for Applications and Species

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