Anti-SHP1 (Tyr-536), Phosphospecific Antibody
Our SHP1 (Tyr-536) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat SHP1 (Tyr-536) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of human Jurkat cells treated with pervanadate (1 mM) for 30 min. The blot was exposed to alkaline phosphatase (lanes 2 & 4) then probed with anti-SHP1 (C-terminal) antibody (lanes 1 & 2) or anti-SHP1 (Tyr-536) antibody (lanes 3-5). The SHP1 (Tyr-536) antibody was used in the presence of phospho-SHP1 (Tyr-536) peptide (lane 5).
Product Specific References for Applications and Species
- Flow Cytometry: Human
- Immunocytochemistry: Human | Mouse
- RPPA: Human
- Western Blot: Human | Mouse | Rat
| Flow Cytometry: Human | ||
| PMID | Dilution | Publication |
| 24405902 | not listed | Le Page, A, et al. 2014. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell communication and signaling, 2. |
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 29307735 | not listed | Le Page, A, et al. 2018. Signal transduction changes in CD4+ and CD8+ T cell subpopulations with aging. Experimental Gerontology, 128-139. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 25876760 | not listed | Cerny, O, et al. 2015. Bordetella pertussis Adenylate Cyclase Toxin Blocks Induction of Bactericidal Nitric Oxide in Macrophages through cAMP-Dependent Activation of the SHP-1 Phosphatase. Journal of immunology (Baltimore, Md. : 1950), 4901-4913. |
| Reverse-Phase Protein Assay: Human | ||
| PMID | Dilution | Publication |
| 29187853 | not listed | Presotto, D, et al. 2017. Fine-Tuning of Optimal TCR Signaling in Tumor-Redirected CD8 T Cells by Distinct TCR Affinity-Mediated Mechanisms. Frontiers in immunology, 1564. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 24405902 | not listed | Le Page, A, et al. 2014. Downregulation of inhibitory SRC homology 2 domain-containing phosphatase-1 (SHP-1) leads to recovery of T cell responses in elderly. Cell communication and signaling, 2. |
| 24713856 | not listed | Kataoka, TR, et al. 2013. CD72 regulates the growth of KIT-mutated leukemia cell line Kasumi-1. Scientific reports, 2861. |
| 23391724 | not listed | Hebeisen, M, et al. 2013. SHP-1 phosphatase activity counteracts increased T cell receptor affinity. The Journal of Clinical Investigation, 1044-1056. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 28811476 | 1:1000 | Mkaddem, SB, et al. 2017. Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. Nature communications, 246. |
| 28250424 | not listed | Choi, S, et al. 2017. THEMIS enhances TCR signaling and enables positive selection by selective inhibition of the phosphatase SHP-1. Nature immunology, 433-441. |
| 27271569 | not listed | Moogk, D, et al. 2016. Constitutive Lck Activity Drives Sensitivity Differences between CD8+ Memory T Cell Subsets. Journal of immunology (Baltimore, Md. : 1950), 644-654. |
| 25239131 | not listed | Kataoka, TR, et al. 2015. CD72 negatively regulates mouse mast cell functions and down-regulates the expression of KIT and FcεRIα. International immunology, 95-103. |
| 24990897 | not listed | Zhou, X, et al. 2014. Leishmania infantum-chagasi activates SHP-1 and reduces NFAT5/TonEBP activity in the mouse kidney inner medulla. American Journal of Physiology. Renal Physiology, F516-524. |
| 19201855 | not listed | Verhagen, AM, et al. 2009. A kinase-dead allele of Lyn attenuates autoimmune disease normally associated with Lyn deficiency. Journal of immunology (Baltimore, Md. : 1950), 2020-2029. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 28017654 | not listed | Park, SY, et al. 2017. Hippocalcin Promotes Neuronal Differentiation and Inhibits Astrocytic Differentiation in Neural Stem Cells. Stem Cell Reports, 95-111. |
| 26342897 | not listed | Choi, YR, et al. 2015. FcγRIIB mediates the inhibitory effect of aggregated α-synuclein on microglial phagocytosis. Neurobiology of Disease, 90-99. |
| 24140598 | not listed | Tibaldi, E, et al. 2014. The tyrosine phosphatase SHP-1 inhibits proliferation of activated hepatic stellate cells by impairing PDGF receptor signaling. Biochimica et Biophysica acta, 288-298. |