Name: Anti-Serotonin Transporter (Thr276) Antibody
Brand: PhosphoSolutions
SKU: p1700-276
Price: 415.0 USD
Availability: InStock

Our Anti-Serotonin Transporter (Thr276) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Serotonin Transporter (Thr276) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Human, Non-Human Primate, Sheep, Xenopus

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

SLC6A4

Molecular Weight

76 kDa

Go to Full Product Details

Western blot of rat mid brain membrane lysate showing specific immunolabeling of the ~76 kDa SERT protein phosphorylated at Thr276 in the first lane (-). Phosphospecificity is shown in the second lane (+) where Immunolabeling is blocked by preadsorption of the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

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Product Details

Target

Serotonin Transporter (Thr276)

Target Description

The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG). cGMP- and PKG-mediated SERT regulation requires phosphorylation at Thr-276 (Ramamoorthy et al., 2007). It has been suggested that although PKG is involved in the stimulation of SERT at Thr-276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012). Also of therapeutic importance, mutation at the Thr-276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

SLC6A4

Molecular Weight

76 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr276 of rat SERT, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Human, Non-Human Primate, Sheep, Xenopus

Antibody Registry ID

AB_2492237

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~76 kDa SERT protein phosphorylated at Thr276. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr276

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

5 HTT antibody, 5 HTTLPR antibody, 5 hydroxytryptamine (serotonin) transporter antibody, 5 hydroxytryptamine transporter antibody, 5HT transporter antibody, 5HTT antibody, hSERT antibody, HTT antibody, Na+/Cl- dependent serotonin transporter antibody, OCD1 antibody, SC6A4_HUMAN antibody, Serotonin transporter 1 antibody, SERT antibody, SERT1 antibody, Slc6a4 antibody, Sodium dependent serotonin transporter antibody, Sodium-dependent serotonin transporter antibody, Solute carrier family 6 (neurotransmitter transporter) member 4 antibody, solute carrier family 6 (neurotransmitter transporter serotonin) member 4 antibody, Solute carrier family 6 member 4 antibody

UniProt Details

UniProt (Human): P31645
UniProt (Immunogen Species): P31652

NCBI Gene ID

25553

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
29486157	not listed	Lu, Q., et al. 2018. Abnormalities of the serotonergic system in diacylglycerol kinase δ-deficient mouse brain. Biochemical and Biophysical Research Communications, 497(4), pp.1031-1037.

Western Blot: Rat
PMID	Dilution	Publication
27140629	1:1000	Zhang, Y.W., et al. 2016. Control of serotonin transporter phosphorylation by conformational state. Proceedings of the National Academy of Sciences, 113(20), E2776-E2783.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Serotonin Transporter (Thr276) Antibody

Our Anti-Serotonin Transporter (Thr276) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Serotonin Transporter (Thr276) and is antigen affinity purified from pooled serum. It is great for use in WB.