Immunoflourescence of HeLa cells labeled with anti-SAP49 (cat. 1900-SAP, red , 1:1000) and anti-vimentin (cat. 2105-VIM, green, 1:10,000). The blue is DAPI staining nuclear DNA.
The spliceosome associated protein of ~49 kDa (SAP49), also known as SF3B4, is a widely expressed nuclear splicing factor that has been shown to have RNA binding activity as well as direct interaction with SAP145 (Champion-Arnaud and Reed 1994). The SAP145-SAP49 complex has recently been implicated in cell cycle progression as Vpr (viral protein 1 of HIV-1) has been shown to bind SAP145 thus interfering with the proper formation and functioning of the SAP145-SAP49 complex (Terada and Yasuda 2006).
Protein G Purified
ICC, IHC, WB
Recombinant full length human SAP49 expressed in and purified from E. Coli.
Bovine, Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Protein G purified culture supernatant.
PBS + 50% glycerol and 5 mm NaN3
Specific for endogenous levels of the ~49 kDa SAP49 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Champion-Arnaud P, Reed R. (1994) The prespliceosome components SAP 49 and SAP 145 interact in a complex implicated in tethering U2 snRNP to the branch site. Genes and Development 8(16):1974-83. PMID: 7958871
Terada Y, Yasuda Y. (2006) Human immunodeficiency virus type 1 Vpr induces G2 checkpoint activation by interacting with the splicing factor SAP145. Molecular and Cellular Biology 26(21):8149-58. PMID: 16923959
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