Anti-PYK2 (Tyr-402), Phosphospecific Antibody (M269)

Name: Anti-PYK2 (Tyr-402), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: PM2691
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Clone

M269

Applications

Host Species

Mouse

Format

Protein A Purified

Gene Name

PTK2B

Molecular Weight

116

Go to Full Product Details

Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-PYK2 (C-terminal region) antibody (lanes 1 & 2) or anti-PYK2 (Tyr-402) (lanes 3 & 4).

Volume: 100 µL

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Product Details

Target

PYK2 (Tyr-402)

Target Description

Protein tyrosine kinase, Pyk2 (CAKb, RAFTK, CADTK), is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration of various cell types. In T-cells, Pyk2 is tyrosine phosphorylated and activated upon ligation of TCR. Phosphorylation of Tyr-402 is required for the phosphorylation of other tyrosines in Pyk2 and provides a binding site for Fyn SH2 during T-cell activation.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M269

Isotype

IgG1

Applications

Host Species

Mouse

Gene Name

PTK2B

Molecular Weight

116

Antigen

Clone M269 was generated from a phosphopeptide containing amino acids surrounding tyrosine 402 in human PYK2. This sequence is conserved in rat and mouse PYK2, and has significant homology to the conserved site in FAK.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 116 kDa* band corresponding to PYK2 on SDS-PAGE immunoblots of human A431, Jurkat and K562 cells treated with pervanadate, but is not observed in control, untreated cells.

Post Translational Modification Type

Phosphorylated

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CAKbeta, FAK2

UniProt Details

UniProt (Human): Q14289
UniProt (Immunogen Species): Q14289

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Anti-PYK2 (Tyr-402), Phosphospecific Antibody (M269)

Anti-PYK2 (Tyr-402), Phosphospecific Antibody
SKU: PM2691

Bulk Order Anti-PYK2 (Tyr-402), Phosphospecific Antibody

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Anti-PYK2 (Tyr-402), Phosphospecific Antibody (M269)

Anti-PYK2 (Tyr-402), Phosphospecific Antibody SKU: PM2691

Bulk Order Anti-PYK2 (Tyr-402), Phosphospecific Antibody

Product Details

Anti-PYK2 (Tyr-402), Phosphospecific Antibody
SKU: PM2691