Western Blot of transfected U20S cell lysate showing specific immunolabeling of PUMA. This antibody detects alpha as well as beta forms of PUMA.
Bulk Order Anti-PUMA Antibody
A novel gene PUMA (p53 upregulated modulator of apoptosis) is a target for activation by the p53 tumor-suppressor gene. p53 functions as a transcriptional activator, and influences p53-inducible genes that play a role in the induction of apoptosis in response to p53. Antisense inhibition of PUMA expression reduced the apoptotic response to p53, and PUMA is likely to play a role in mediating p53-induced cell death through the cytochrome c/Apaf-1-dependent pathway.
Antigen Affinity Purified
Synthetic peptide corresponding to amino acids from a sequence found in the exon-3-encoded region in the human PUMA-a and PUMA-b forms of the protein.
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
PBS + 0.02% NaN3
WB Brain: 1:500-1:1000
Reacts with a sequence found in the exon-3-encoded region in the human PUMA-a and PUMA-b forms of the protein. Recognizes both forms of the protein
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.