Name: Anti-Progesterone Receptor (Ser190) Antibody
Brand: PhosphoSolutions
SKU: p205-190
Price: 415.0 USD
Availability: InStock

Our Anti-Progesterone Receptor (Ser190) phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Progesterone Receptor (Ser190) and is Protein G purified. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human

Clone

1154

Applications

IHC, WB

Host Species

Mouse

Format

Protein G Purified

Gene Name

PGR

Molecular Weight

90/120 kDa

Go to Full Product Details

Western blot of T47D cell lysate prepared from cells that had been incubated in the presence of the synthetic progestin agonist R5020 (500 nM) showing specific immunolabeling of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform of the progesterone receptor phosphorylated at Ser190. The immunolabeling is specifically blocked by the phosphopeptide used as the antigen (not shown).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Progesterone Receptor (Ser190) Antibody

Product Details

Target

Progesterone Receptor (Ser190)

Target Description

There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-A and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the A and B forms of the receptor (Clemm et al., 2000) . Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser-190 and Ser-294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor.

Format

Protein G Purified

Clonality

Monoclonal

Clone

1154

Isotype

IgG1

Applications

IHC, WB

Host Species

Mouse

Gene Name

PGR

Molecular Weight

90/120 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser190 of human progesterone receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human

Antibody Registry ID

AB_2492215

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~90 kDa PR-A isoform and the ~120 kDa PR-B isoform phosphorylated at Ser190. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser190

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

NR3C3 antibody, Nuclear receptor subfamily 3 group C member 3 antibody, PGR antibody, PR antibody, PRA antibody, PRB antibody, PRGR_HUMAN antibody, Progesterone receptor antibody, Progestin receptor form A antibody, Progestin receptor form B antibody

UniProt Details

UniProt (Human): P06401
UniProt (Immunogen Species): P06401

NCBI Gene ID

5241

Product Specific Protocols

Western Blot Protocol: Download

General References

Attia, G.R., Zeitoun, K., Edwards, D., Johns, A., Carr, B.R. and Bulun, S.E., 2000. Progesterone receptor isoform A but not B is expressed in endometriosis. The Journal of Clinical Endocrinology & Metabolism, 85(8), pp.2897-2902. PMID: 10946900
Clemm, D.L., Sherman, L., Boonyaratanakornkit, V., Schrader, W.T., Weigel, N.L. and Edwards, D.P., 2000. Differential hormone-dependent phosphorylation of progesterone receptor A and B forms revealed by a phosphoserine site-specific monoclonal antibody. Molecular Endocrinology, 14(1), pp.52-65.PMID: 10628747
Edwards, D.P., 2005. Regulation of signal transduction pathways by estrogen and progesterone. Annual Review ofPhysiology, 67, pp.335-376. PMID: 15709962
Lin, V.C., Woon, C.T., Aw, S.E. and Guo, C., 2003. Distinct molecular pathways mediate progesterone-induced growth inhibition and focal adhesion. Endocrinology, 144(12), pp.5650-5657. PMID: 12970168

Recover password

Create my account

Anti-Progesterone Receptor (Ser190) Antibody (1154)

Our Anti-Progesterone Receptor (Ser190) phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Progesterone Receptor (Ser190) and is Protein G purified. It is great for use in WB, IHC.

Anti-Progesterone Receptor (Ser190) Antibody
SKU: p205-190

Bulk Order Anti-Progesterone Receptor (Ser190) Antibody

Product Details

Product Specific Protocols

General References

Related Products

Recently Viewed

Anti-Progesterone Receptor (Ser190) Antibody (1154)

Our Anti-Progesterone Receptor (Ser190) phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Progesterone Receptor (Ser190) and is Protein G purified. It is great for use in WB, IHC.

Anti-Progesterone Receptor (Ser190) Antibody SKU: p205-190

Bulk Order Anti-Progesterone Receptor (Ser190) Antibody

Product Details

Product Specific Protocols

General References

Anti-Progesterone Receptor (Ser190) Antibody
SKU: p205-190