Name: Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody
Brand: PhosphoSolutions
SKU: p1551-1007
Price: 415.0 USD
Availability: InStock

Our Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Potassium Chloride Cotransporter (KCC2) (Thr1007) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Human, Non-Human Primate, Sheep

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

KCC2

Molecular Weight

135 kDa

Go to Full Product Details

Western blot of rat hippocampal lysate showing specific labeling of the ~135 kDa KCC2 protein phosphorylated at Thr1007 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody

Product Details

Target

Potassium Chloride Cotransporter (KCC2) (Thr1007)

Target Description

KCC2 is widely thought to be expressed exclusively in neurons where it is responsible for maintaining low intracellular chloride concentration to drive hyperpolarizing post-synaptic responses to the inhibitory neurotransmitters GABA and glycine (Lee et al., 2007). KCC2 is expressed in most adult neurons, and expression levels correlate well with the maturation state of neurons. N-ethylmaleimide (NEM) has been show to increase phosphorylation of the Ser-940 residue, while decreasing phosphorylation of Thr-1007 residue. Dephosphorylation of residues Thr-906 and Thr-1007 correlates with increased KCC2 activity (Moss et al., 2017).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

KCC2

Molecular Weight

135 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr1007 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Human, Non-Human Primate, Sheep

Antibody Registry ID

AB_2716769

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Thr1007. Immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr1007

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Electroneutral potassium chloride cotransporter 2 antibody, Electroneutral potassium-chloride cotransporter 2 antibody, Erythroid K Cl cotransporter 2 antibody, Furosemide sensitive K Cl cotransporter antibody, hKCC2 antibody, K-Cl cotransporter 2 antibody, KCC 2 antibody, KCC2 antibody, KIAA1176 antibody, Neuronal K Cl cotransporter antibody, Neuronal K-Cl cotransporter antibody, Potassium Chloride Cotransporter antibody, Potassium chloride transporter 5 antibody, rKCC2 antibody, S12A5 antibody, S12A5_HUMAN antibody, SLC12A5 antibody, Solute carrier family 12 (potassium chloride transporter) member 5 antibody, Solute carrier family 12 member 5 antibody

UniProt Details

UniProt (Human): Q9H2X9
UniProt (Immunogen Species): Q63633

NCBI Gene ID

171373

Product Specific References for Applications and Species

Western Blot: Mouse | Rat

Western Blot: Mouse
PMID	Dilution	Publication
36973513	not listed	Hu, J.J., et al. 2023. Emergence of Consciousness from Anesthesia Through Ubiquitin Degradation of KCC2 in the Ventral Posteromedial Nucleus of the Thalamus. Nature Neuroscience, .
36889319	1:750	Jarvis, R., et al. 2023. Direct Activation of KCC2 Arrests Benzodiazepine Refractory Status Epilepticus and Limits the Subsequent Neuronal Injury in Mice. Cell Reports Medicine, 100957.

Western Blot: Rat
PMID	Dilution	Publication
29092909	1:1000	Conway, L.C., et al. 2017. N-Ethylmaleimide increases KCC2 cotransporter activity by modulating transporter phosphorylation. Journal of Biological Chemistry, 292(52), pp.21253-21263.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Potassium Chloride Cotransporter (KCC2) (Thr1007) Antibody