Anti-Plexin D1 (Cytoplasmic domain) Antibody

Name: Anti-Plexin D1 (Cytoplasmic domain) Antibody
Brand: PhosphoSolutions
SKU: PP4401
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions Datasheet

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

PLXND1

Molecular Weight

195/215/250

Go to Full Product Details

Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6).

Volume: 100 µL

Variant

Price:

$309

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Bulk Order Anti-Plexin D1 (Cytoplasmic domain) Antibody

Product Details

Target

Plexin D1 (Cytoplasmic domain)

Target Description

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. Plexin D1 is a type I transmembrane protein that may be expressed as multiple isoforms in many cell types, including neurons and endothelial cells. Semaphorin 3E (Sema-3E) and semaphorin 4A can bind Plexin D1, and ligand binding leads to phosphorylation of Tyr-1642 and activation of Plexin D1. Sema-3E and its receptor are important for angiogenesis that occurs during blood vessel development and repair. In cancers, Sema-3E and Plexin D1 are expressed in tumor tissues and cancer cells, and the interaction of these molecules may promote cancer cell migration and metastic spreading. Thus, Plexin D1, and its ligand Sema-3E, may be important regulators of angiogenesis and metastasis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

PLXND1

Molecular Weight

195/215/250

Antigen

Plexin D1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the cytoplasmic domain from human Plexin D1. This sequence is well conserved in rat and mouse Plexin D1, and has low homology to other Plexin family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects 250, 215, and 195 kDa bands corresponding to the apparent molecular mass of Plexin D1 variants in human umbilical vein endothelial cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PLXND1, Sema3E

UniProt Details

UniProt (Human): Q9Y4D7
UniProt (Immunogen Species): Q9Y4D7

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Anti-Plexin D1 (Cytoplasmic domain) Antibody