Anti-Plexin B1 (C-terminal region) Antibody

Name: Anti-Plexin B1 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: PP1841
Price: 329.0 USD
Availability: InStock

2 Citations

Our Plexin B1 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Plexin B1 (C-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling in rat PC12 cells differentiated with NGF. The cells were probed with rabbit polyclonal Plexin B1 (PP1841) and mouse monoclonal Sema-4D (SM1881) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot of mouse liver (lane 1) and rat PC12 cells (lane 2). The blot was probed with rabbit polyclonal Plexin B1 antibody (PP1841) at a dilution of 1:1000.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

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SKU: PP1841

Product Details

Target

Plexin B1 (C-terminal region)

Target Description

Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. The extracellular region of plexins contains a semaphorin domain, multiple glycine-rich motifs, and MET-related sequences. The cytoplasmic region contains a Sex/Plexin domain and putative tyrosine phosphorylation sites that mediate signal transduction after activation. In addition, Plexin B1 and B2 contain cleavage sites for subtilisin-like proprotein convertases. These cleavage sites may be important for regulating assembly of heterodimeric receptors or release of the extracellular domain. Plexin-B1 is expressed as a 300 kDa precursor protein that can be cleaved into a 200 kDa a-subunit and a 100 kDa b-subunit. This plexin is the receptor for the class IV semaphorin, Sema-4D. Sema-4D activation of Plexin B1 recruits and activates c-Met, which can promote cell survival, migration, and angiogenesis

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

PLXNB1

Molecular Weight

100/150/300

Antigen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of human Plexin B1. This sequence has high homology with similar regions in rat and mouse Plexin B1, and has low homology to other plexin family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:400

Conjugate

Unconjugated

Specificity

The antibody detects 300, 150, and 100 kDa* bands on SDS-PAGE immunoblots of mouse liver and rat PC12 cells. These bands correspond to the full length and proteolytically processed forms of Plexin B1. In mouse liver, this antibody detects 300 and 100 kDa forms of Plexin B1.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Sema4D, CD100

UniProt Details

UniProt (Human): O43157
UniProt (Immunogen Species): O43157

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
31861264	not listed	Williamson, M, et al. 2019. PlexinB1 Promotes Nuclear Translocation of the Glucocorticoid Receptor. Cells, 3.

Western Blot: Human
PMID	Dilution	Publication
37563360	not listed	Garg, R, et al. 2023. B-type Plexins promote the GTPase activity of Ran to affect androgen receptor nuclear translocation in prostate cancer. Cancer gene therapy, .

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Anti-Plexin B1 (C-terminal region) Antibody

SKU: PP1841

Product Details

Product Specific References for Applications and Species

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