Anti-Phosphothreonine Antibody

Name: Anti-Phosphothreonine Antibody
Brand: PhosphoSolutions
SKU: PP4641
Price: 275.0 USD
Availability: InStock

Our Anti-Phosphothreonine rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Anti-Phosphothreonine and is antigen affinity purified. It is great for use in WB, ICC, IP.

Immunocytochemical labeling of phosphothreonine upregulation in control (left) or calyculin A-treated HeLa cells (right). The cells were labeled with rabbit polyclonal anti-Phosphothreonine (PP4641). The antibody was detected using goat anti-rabbit DyLight® 594.

Western blot of human A431 cells treated with Calyculin A (100 nM) for 30 min. The blot was untreated (lane 1) or treated with lambda phosphatase (lanes 2), then probed with anti-Phosphothreonine (PP4641) at 1:1000.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Rabbit

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SKU: PP4641

Product Details

Target

Anti-Phosphothreonine

Target Description

Phosphorylation of specific serine or threonine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor ser/thr kinases that mediate these protein modifications. Antibodies that can detect phosphoserine or phosphothreonine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphorylated proteins. Immunoprecipitation of proteins of interest followed by detection of phosphoserine or phosphothreonine using anti-phosphoserine antibody is commonly used to correlate changes in phosphorylation state with alterations in protein activity

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IP, WB

Host Species

Rabbit

Gene Name

N/A

Molecular Weight

N/A

Antigen

Phosphothreonine synthetic peptides coupled to carrier protein.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:250
IP: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unphosphorylated peptide then affinity purified using phospho-threonine peptide (without carrier). The antibody detects many serine or threonine phosphorylated proteins by western blot and ELISA.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

pThr

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Phosphoser/thr mAb

UniProt Details

UniProt (Immunogen Species): N/A

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Anti-Phosphothreonine Antibody

SKU: PP4641

Product Details

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