Anti-PDK1 (C-terminal region) Antibody (M146)

Name: Anti-PDK1 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: PM1461
Price: 275.0 USD
Availability: InStock

Our PDK1 (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat PDK1 (C-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

Western blot image of mouse brain (lane 1) and human A431 cells (lane 2). The blot was probed with anti-PDK1 (C-terminal region) (PM1461).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

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from PhosphoSolutions

SKU: PM1461

Volume: 100 µL

Variant

Price:

$275

Quantity:

Product Details

Target

PDK1 (C-terminal region)

Target Description

3-Phosphoinositide-dependent kinase 1 (PDK1), also known as PKB kinase, was identified as the activator of the survival kinase Akt/PKB. Several important substrates of PDK1 include p70S6 kinase, PKAs, PKCs, SGKs, RSKs, and PAKs. PDK1 is a member of the AGC superfamily of serine/threonine kinases. Through the phosphorylation of downstream kinases, like Akt, PDK1 has been shown to be involved in several different cell functions, such as protein synthesis, cell survival, glucose metabolism, and cell adhesion and migration. The regulation of PDK1 occurs through lipid second messengers and phosphorylation. Multiple serine sites are phosphorylated on PDK1. Serine 241 phosphorylation is required for PDK1 activity, while serine 396 has been implicated in PDK1 nuclear translocation. Tyrosine phosphorylation may also regulate PDK1 activity. Tyrosines 9 and 373/376 are phosphorylated by c-Src in vitro. Tyr-373/Tyr-376 are important for PDK1 activity, while Tyr-9 phosphorylation permits Tyr-373/Tyr-376 phosphorylation by c-Src. In addition, Tyr-9 may be important during angiotensin-II-induced focal adhesion formation.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M146

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

PDK1

Molecular Weight

Antigen

Clone M146 was generated from a recombinant protein containing amino acid residues in the C-terminal region of human PDK1. This sequence is highly conserved in rat and mouse PDK1

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:250
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 65 kDa* protein corresponding to the apparent molecular mass of PDK1 on SDS-PAGE immunoblots of human A431 cells and mouse brain.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

PDPK1, PKBK

UniProt Details

UniProt (Human): O15530
UniProt (Immunogen Species): O15530

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Anti-PDK1 (C-terminal region) Antibody (M146)

Our PDK1 (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat PDK1 (C-terminal region) and is Protein A purified. It is great for use in WB, ICC.

SKU: PM1461

Product Details

Bulk Order Anti-PDK1 (C-terminal region) Antibody

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