Anti-Paxillin (Tyr-118), Phosphospecific Antibody

Name: Anti-Paxillin (Tyr-118), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: PP4501
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

6 Citations

Product Type

Primary Antibody

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

PXN

Molecular Weight

Go to Full Product Details

Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with mouse monoclonal anti-Paxillin (PM1071) (lanes 1 & 2) and rabbit polyclonal anti-phospho-Paxillin (Tyr-118) (PP4501) (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$309

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Product Details

Target

Paxillin (Tyr-118)

Target Description

Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr-31 and Tyr-118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

PXN

Molecular Weight

Antigen

Phospho-Paxillin (Tyr-118) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 118 from human paxillin. This sequence is highly conserved in rat and mouse paxillin and is also found in all isoforms (a, b, g) of paxillin.

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

This antibody detects a 68kDa* protein corresponding to the molecular mass of paxillin on SDS-PAGE immunoblots of A431 cells treated with pervanadate. This reactivity is not observed after akaline phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr 118

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): P49023
UniProt (Immunogen Species): P49023

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
33007249	1:500	Juanes, MA, et al. 2020. EB1 Directly Regulates APC-Mediated Actin Nucleation. Current biology : CB, 4763-4772.
31471457	1:500	Juanes, MA, et al. 2019. The role of APC-mediated actin assembly in microtubule capture and focal adhesion turnover. The Journal of cell biology, 3415-3435.
28663347	1:1000	Juanes, MA, et al. 2017. Adenomatous polyposis coli nucleates actin assembly to drive cell migration and microtubule-induced focal adhesion turnover. The Journal of cell biology, 2859-2875.

Immunocytochemistry: Rat
PMID	Dilution	Publication
29271742	1:100	Chang, TY, et al. 2017. Paxillin facilitates timely neurite initiation on soft-substrate environments by interacting with the endocytic machinery. eLife, e31101.

Western Blot: Human
PMID	Dilution	Publication
28663347	1:500	Juanes, MA, et al. 2017. Adenomatous polyposis coli nucleates actin assembly to drive cell migration and microtubule-induced focal adhesion turnover. The Journal of cell biology, 2859-2875.

Western Blot: Mouse
PMID	Dilution	Publication
37470177	1:1000	Tachibana, H, et al. 2023. Plasma Membrane of Focal Adhesions has a High Content of Cholesterol and Phosphatidylcholine with Saturated Acyl Chains. Journal of cell science, jcs260763.

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Anti-Paxillin (Tyr-118), Phosphospecific Antibody