Anti-Paxillin (Ser-178), Phosphospecific Antibody

Name: Anti-Paxillin (Ser-178), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: PP1051
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

PXN

Molecular Weight

Go to Full Product Details

Western blot analysis of A431 cells (20 µg/lane) serum starved overnight and treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-Paxillin mouse monoclonal (PM1071) or anti-Paxillin (Ser-178) rabbit polyclonal (PP1051).

Volume: 100 µL

Variant

Price:

$309

Quantity:

Bulk Order Anti-Paxillin (Ser-178), Phosphospecific Antibody

Product Details

Target

Paxillin (Ser-178)

Target Description

Paxillin, a focal adhesion protein, is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and SH3-/SH2-binding domains that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Serine phosphorylation of paxillin occurs in response to growth-factor activation and fibronectins. Both JNK1 and cdc2 kinases can phosphorylate serine 178 in paxillin. The mutant form of paxillin (S178A) decreases the migration of keratocytes and epithelial cells. Thus, phosphorylation paxillin at serine 178 may be important during cell migration

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

PXN

Molecular Weight

Antigen

Phospho-Paxillin (Ser-178) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 178 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:500
IHC: 1:100

Conjugate

Unconjugated

Specificity

This antibody detects a 68kDa* protein corresponding to the molecular mass of phosphorylated paxillin on SDS-PAGE immunoblots of EGF treated A431 cells, but not in A431 control cells. Similar results were seen in calyculin A treated human A431 and aortic endothelial cells.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-178

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

UniProt Details

UniProt (Human): P49023
UniProt (Immunogen Species): P49023

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Anti-Paxillin (Ser-178), Phosphospecific Antibody