Name: Anti-PanK4 (Thr63) Antibody
Brand: PhosphoSolutions
SKU: p188-63
Price: 415.0 USD
Availability: InStock

Our Anti-PanK4 (Thr63) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse PanK4 (Thr63) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Mouse

Expected Reactivity

Hamster, Rat

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

PANK4

Molecular Weight

86 kDa

Go to Full Product Details

Western blot of mouse whole brain lysate showing specific labeling of the ~86 kDa PanK4 phosphorylated at Ser63 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is nearly eliminated with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-PanK4 (Thr63) Antibody

Product Details

Target

PanK4 (Thr63)

Target Description

Pantothenate kinase, PanK, is a vital regulatory enzyme for coenzyme A (CoA) biosynthesis, phosphorylating pantothenate (vitamin B5) to 4’-phosphopantothenate, then quickly transforming to CoA which is an essential component for fatty acid metabolism (Abiko, Y, 1967). There are 4 members of the PanK family, located on chromosomes 10q23.31, 20p13, 5q35, and 1p36.32 (Zhou et al, 2001). PanK1 is predominantly in heart, liver, and kidney. PanK2 is expressed ubiquitously, with higher levels in retinal and infant basal ganglia. PanK3 has high levels in liver, while PanK4 is expressed ubiquitously with its highest levels found in muscle (Zhou et al, 2001). Additionally, PanK4 has been shown to regulate Pkm2 activity affecting glucose metabolism (Li et al, 2005). There have been several phospho-serine, threonine, and tyrosine sites identified within PanK4, the role of each one has yet to be determined.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

PANK4

Molecular Weight

86 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr63 of mouse PanK4, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse

Expected Reactivity

Hamster, Rat

Antibody Registry ID

AB_2636991

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for the ~86 kDa PanK4 phosphorylated at Ser63. Immunolabeling of PanK4 is nearly eliminated with λ-phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Thr63

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

DKFZp547M242 antibody, FLJ10782 antibody, hPanK 4 antibody, hPanK4 antibody, PANK 4 antibody, Pantothenate kinase 4 antibody, Pantothenic acid kinase antibody

UniProt Details

UniProt (Immunogen Species): Q80YV4

NCBI Gene ID

269614

Product Specific Protocols

Western Blot Protocol: Download

General References

Abiko, Y. Investigations on pantothenic acid and its related compounds. IX. Biochemical Studies. 4. Separation and substrate specificity of pantothenate kinase and phosphopantothenoylcysteine synthetase. J. Biochem (Tokyo) 61, 290-299 (1967). PMID: 6053309
Zhou B, Westaway S, Levinson B, Johnson M, Gitschier J, Hayflick S. A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome.Nat Genet 28, 345-349 (2001). PMID: 11479594
Li Y, Chang Y, Zhang L, Feng Q, Liu Z, Zhang Y, Zuo J, Meng Y, Fang F. High glucose upregulates pantothenate kinase 4 (PanK4) and thus affects M2-type pyruvate kinase (Pkm2).Mol Cell Biochem 277, 117-125 (2005). PMID: 16132722

Recover password

Create my account

Anti-PanK4 (Thr63) Antibody

Our Anti-PanK4 (Thr63) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse PanK4 (Thr63) and is antigen affinity purified from pooled serum. It is great for use in WB.