Anti-Pan-Neurofascin (Extracellular) Antibody (A12/18)
Primary Antibody
Human, Rat
A12/18
IHC, IP, WB
Mouse
Purified
Nfasc
155, 186 kDa
![immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.](http://www.phosphosolutions.com/cdn/shop/products/validation-image-73-172-a12-18-wb2_2ab63250-3d23-4fa1-b486-84232eb7dbb9_{width}x.jpg?v=1626903206)
immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.
![immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.](http://www.phosphosolutions.com/cdn/shop/products/validation-image-73-172-a12-18-wb2_2ab63250-3d23-4fa1-b486-84232eb7dbb9_1024x.jpg?v=1626903206)
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Non-Human Primate | Rat
- Immunohistochemistry: Human | Rat
- Live Cell Imaging: Rat
- Pulsed-pH Live Imaging: Rat
- Western Blot: Human | Mouse | Rat
- Additional Publications: Unspecified
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 35314674 | 1:100 | Keren-Kaplan, T., et al. 2022. RUFY3 and RUFY4 are ARL8 effectors that promote coupling of endolysosomes to dynein-dynactin. Nature Communications, 1506. |
| 31100061 | 1:50 (supe) | Guardia, C. M., et al. 2019. Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning. PLoS Biology, e3000279. |
| 26671463 | not listed | van Beuningen, S., et al. 2015. TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. Neuron, 1208-26. |
| 25283719 | 1:50-1:100 | Notturno, F., et al. 2014. Autoantibodies to neurofascin-186 and gliomedin in multifocal motor neuropathy.. Journal of Neuroimmunology, 207-12. |
| 24859753 | 1:100 | Lukinavičius, G., et al. 2014. Fluorogenic probes for live-cell imaging of the cytoskeleton.. Nature Methods, 731-3. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 35978188 | 1:500 | Eichel, K., et al. 2022. Endocytosis in the axon initial segment maintains neuronal polarity. Nature, 128-135. |
| 31328333 | 1:100 | Dubessy, A. L., et al. 2019. Role of a Contactin multi‐molecular complex secreted by oligodendrocytes in nodal protein clustering in the CNS. Glia, 2248-63. |
| 27098687 | 1:500 | Fréal, A., et al. 2016. Cooperative Interactions between 480 kDa Ankyrin-G and EB Proteins Assemble the Axon Initial Segment. Journal of Neuroscience, 4421-33. |
| 26947559 | 1:200 | D'Este, E., et al. 2016. Subcortical cytoskeleton periodicity throughout the nervous system. Scientific Reports, 22741. |
| 25950943 | not listed | Papandréou, M. J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-37. |
| Immunocytochemistry: Non-Human Primate | ||
| PMID | Dilution | Publication |
| 31100061 | 1:50 (supe) | Guardia, C. M., et al. 2019. Reversible association with motor proteins (RAMP): A streptavidin-based method to manipulate organelle positioning. PLoS Biology, e3000279. |
| 22649224 | not listed | Yap, C. C., et al. 2012. Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding.. Journal of Neuroscience, 7439-53. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 33571451 | not listed | Zahavi, E. E., et al. 2021. Combined kinesin-1 and kinesin-3 activity drives axonal trafficking of TrkB receptors in Rab6 carriers. Developmental Cell, 494-508.e7. |
| 32958558 | not listed | Collura, K. M., et al. 2020. The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway. Journal of Biological Chemistry, 15427-37. |
| 32553155 | 1:1000 | De Pace, R., et al. 2020. Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons. Cell Reports, 107775. |
| 32239080 | not listed | Gerasimaitė, R., et al. 2020. Efflux pump insensitive rhodamine-jasplakinolide conjugates for G- and F-actin imaging in living cells. Organic & Biomolecular Chemistry, 2929-37. |
| 31882453 | 1:100 | Gaub, B. M., et al. 2020. Neurons differentiate magnitude and location of mechanical stimuli. Proceedings of the National Academy of Sciences, 848-856. |
| 31727776 | not listed | Torii, T., et al. 2020. NuMA1 promotes axon initial segment assembly through inhibition of endocytosis. Journal of Cell Biology, e201907048. |
| 31658245 | not listed | Ribeiro, L. F., et al. 2019. SorCS1-mediated sorting in dendrites maintains neurexin axonal surface polarization required for synaptic function. PLoS Biology, e3000466. |
| 30967428 | 1:200 | Harterink, M., et al. 2019. TRIM46 Organizes Microtubule Fasciculation in the Axon Initial Segment. Journal of Neuroscience, 4864-73. |
| 30772082 | not listed | Farías, G. G., et al. 2019. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity. Neuron, 184-201.e8. |
| 30598502 | not listed | Hivert, B., et al. 2019. ADAM22 and ADAM23 modulate the targeting of the Kv1 channel-associated protein LGI1 to the axon initial segment. . Journal of Cell Science, jcs219774. |
| 30016622 | 1ug/ml | Karasmanis, E.P., et al. 2018. Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin. Developmental Cell, 204-218. |
| 29395909 | not listed | Berger, S.L., et al. 2018. Localized Myosin II Activity Regulates Assembly and Plasticity of the Axon Initial Segment. Neuron, 555-570. |
| 28828584 | not listed | Guo, Y., et al. 2017. Neurons with Multiple Axons Have Functional Axon Initial Segments. Neuroscience Bulletin, 641-52. |
| 28559423 | not listed | Ganguly, A., et al. 2017. Hsc70 chaperone activity is required for the cytosolic slow axonal transport of synapsin. Journal of Cell Biology, 2059-74. |
| 28533267 | not listed | Pinatel, D., et al. 2017. The Kv1-associated molecules TAG-1 and Caspr2 are selectively targeted to the axon initial segment in hippocampal neurons. Journal of Cell Science, 2209-2220. |
| 28394337 | not listed | Harterink, M., et al. 2017. DeActs: genetically encoded tools for perturbing the actin cytoskeleton in single cells. Nature Methods, 479-82. |
| 27932952 | not listed | Dumitrescu, A. S., et al. 2016. Evaluating Tools for Live Imaging of Structural Plasticity at the Axon Initial Segment. Frontiers in Cellular Neuroscience, 268. |
| 27697928 | not listed | Albrecht, D., et al. 2016. Nanoscopic compartmentalization of membrane protein motion at the axon initial segment.. The Journal of Biology, 37-46. |
| 27559088 | not listed | Guo, X., et al. 2016. Rab5 and its effector FHF contribute to neuronal polarity through dynein-dependent retrieval of somatodendritic proteins from the axon. Proceedings of the National Academy of Sciences, E5318-27. |
| 27356871 | not listed | Sohn, P. D., et al. 2016. Acetylated tau destabilizes the cytoskeleton in the axon initial segment and is mislocalized to the somatodendritic compartment.. Molecular Neurodegeneration, 47. |
| 27220554 | 1:400 | Sidenstein, S. C., et al. 2016. Multicolour Multilevel STED nanoscopy of Actin/Spectrin Organization at Synapses.. Scientific Reports, 26725. |
| 26888935 | 1:100 | Benned-Jensen, T., et al. 2016. Live Imaging of Kv7.2/7.3 Cell Surface Dynamics at the Axon Initial Segment: High Steady-State Stability and Calpain-Dependent Excitotoxic Downregulation Revealed.. Journal of Neuroscience, 2261-6. |
| 26553875 | 1:100 | Norkett, R., et al. 2016. DISC1-dependent Regulation of Mitochondrial Dynamics Controls the Morphogenesis of Complex Neuronal Dendrites.. The Journal of Biological Chemistry, 613-29. |
| 26526995 | not listed | Evans, M. D., et al. 2015. Rapid Modulation of Axon Initial Segment Length Influences Repetitive Spike Firing.. Cell Report, 1233-45. |
| 26216902 | not listed | Ganguly, A., et al. 2015. A dynamic formin-dependent deep F-actin network in axons.. The Journal of Biology, 401-417. |
| 26019348 | 1:200 | Franssen, E. H., et al. 2015. Exclusion of integrins from CNS axons is regulated by Arf6 activation and the AIS.. Journal of Neuroscience, 8359-75. |
| 25950943 | not listed | Papandréou, M. J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-37. |
| 25561543 | not listed | Freeman, S.A., et al. 2015. Acceleration of conduction velocity linked to clustering of nodal components precedes myelination. . Proceedings of the National Academy of Sciences, E321-E328. |
| 24959118 | not listed | Muir, J., et al. 2014. Plasticity of GABAA receptor diffusion dynamics at the axon initial segment.. Frontiers in Neuroscience, 151. |
| 24711503 | 1:100 | Jones, S. L., et al. 2014. Axon initial segment cytoskeleton comprises a multiprotein submembranous coat containing sparse actin filaments.. The Journal of Biology, 67-81. |
| 22649224 | not listed | Yap, C. C., et al. 2012. Doublecortin (DCX) mediates endocytosis of neurofascin independently of microtubule binding.. Journal of Neuroscience, 7439-53. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 32289157 | 1:3000 | Bekku, Y., et al. 2020. Independent anterograde transport and retrograde cotransport of domain components of myelinated axons. Journal of Cell Biology, e201906071. |
| 26671463 | not listed | van Beuningen, S., et al. 2015. TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. Neuron, 1208-26. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 30772082 | not listed | Farías, G. G., et al. 2019. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity. Neuron, 184-201.e8. |
| Live Cell Imaging: Rat | ||
| PMID | Dilution | Publication |
| 36859689 | not listed | Christensen, K.R., et al. 2023. Phosphomimetics at Ser199/Ser202/Thr205 in Tau Impairs Axonal Transport in Rat Hippocampal Neurons. Molecular Neurobiology, . |
| Pulsed-pH Live Imaging: Rat | ||
| PMID | Dilution | Publication |
| 35978188 | 1:250 | Eichel, K., et al. 2022. Endocytosis in the axon initial segment maintains neuronal polarity. Nature, 128-135. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 31900387 | 1:500 | Hamdan, H., et al. 2020. Mapping axon initial segment structure and function by multiplexed proximity biotinylation. Nature Communications, 100. |
| 25378584 | not listed | Jain, S., et al. 2015. Polarized sorting of the copper transporter ATP7B in neurons mediated by recognition of a dileucine signal by AP-1.. Molecular Biology of the Cell, 218-28. |
| 22473675 | not listed | Dhaunchak, A.S., et al. 2012. Implication of perturbed axoglial apparatus in early pediatric multiple sclerosis.. Annals of Neurology, 601-13. |
| 20830807 | not listed | Dhaunchak, A.S., et al. 2010. A proteome map of axoglial specializations isolated and purified from human central nervous system.. Glia, 1949-60. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 36085162 | 1:10,000 | Hatayama, M., et al. 2022. SLITRK1-Mediated Noradrenergic Projection Suppression in the Neonatal Prefrontal Cortex. Communications Biology, 935. |
| 31910261 | not listed | Lee, M., et al. 2020. Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain. Journal of Cell Biology, e201903033. |
| 27098687 | 1:500 | Fréal, A., et al. 2016. Cooperative Interactions between 480 kDa Ankyrin-G and EB Proteins Assemble the Axon Initial Segment. Journal of Neuroscience, 4421-33. |
| 25950943 | not listed | Papandréou, M. J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-37. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 28785724 | 1:1000 | Kneynsberg, A., et al. 2017. Aging Does Not Affect Axon Initial Segment Structure and Somatic Localization of Tau Protein in Hippocampal Neurons of Fischer 344 Rats. eNeuro, ENEURO.0043-17.2017. |
| 25950943 | not listed | Papandréou, M. J., et al. 2015. CK2-regulated schwannomin-interacting protein IQCJ-SCHIP-1 association with AnkG contributes to the maintenance of the axon initial segment.. Journal of Neurochemistry, 527-37. |
| Additional Publications: Unspecified | ||
| PMID | Publication | |
| 27631998 | Farías, G. G., et al. 2016. Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons. Methods in Molecular Biology, 13-30. | |
| 25732815 | D'Este, E., et al. 2015. STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons.. Cell Report, 1246-51. | |
| 22632975 | Galiano, M.R., et al. 2012. A distal axonal cytoskeleton forms an intra-axonal boundary that controls axon initial segment assembly.. Cell, 1125-39. | |
| 19001126 | Hedstrom, K.L., et al. 2008. AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity.. The Journal of Biology, 635-40. | |