immunoblot on crude membrane fractions from whole mouse (MBM) and rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] and cerebellum [HBM(Cb)] probed with A12/18 (left) and N52A/42 (right) TC supe.
Anti-Pan-Neurofascin (Extracellular) Antibody
Neurofascin is encoded by the gene NFASC. Two major isoforms of NeuroFascin are expressed as a result of alternative splicing, NF186 and NF155. These proteins are expressed in the central nervous system. In brain, NF186 is expressed at Nodes of Ranvier andNF155 is expressed in unmyelinated axons. Neuofascin plays a role in cell adhesion and is thought to be involved in processes such as neurite extension, synaptogenesis and myelination. Diseases associated with this gene include Demyelinating Polyneuropathy.
IHC, IP, WB
155, 186 kDa
Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin by: - binding to NF-186-tranfected COS cells- recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM- immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry - loss of immunostaining against NF186-shRNA knockdown samples Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186); Mouse: 98% identity (1074/1086 amino acids identical); Human: 79% identity (955/1195 amino acids identical); This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.