Name: Anti-p53 (Ser392) Antibody
Brand: PhosphoSolutions
SKU: p195-392
Price: 415.0 USD
Availability: InStock

Our Anti-p53 (Ser392) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat p53 (Ser392) and is antigen affinity purified from pooled serum. It is great for use in WB.

Product Type

Primary Antibody

Species Reactivity

Rat

Applications

WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

TP53

Molecular Weight

53 kDa

Go to Full Product Details

$Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53 kDa p53 phosphorylated at Ser392 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).$

Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53 kDa p53 phosphorylated at Ser392 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).

$Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53 kDa p53 phosphorylated at Ser392 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).$

Volume: 100 µL

Variant

Price:

$415

Quantity:

Bulk Order Anti-p53 (Ser392) Antibody

Product Details

Target

p53 (Ser392)

Target Description

p53 has a well established role in blocking the proliferative action of damaged cells and acting in essence as an anticancer agent (Sharpless and DePinho, 2002; Yin et al., 1992). It has been called the guardian of the genome (Lane, 1992). Phosphorylation of Ser-392 in p53 is associated with formation of human tumors (Saito et al., 2003; Pise-Masison et al., 1998; Kim et al., 2004). In addition, p53 has been linked to effects of aging and oxidative stress (Sharpless and DePinho, 2002). An increase in p53 has also been linked to deficits in LTP and learning and memory (Jiang et al., 1998)

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

TP53

Molecular Weight

53 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392 of human p53, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Rat

Antibody Registry ID

AB_2492196

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~53 kDa p53 protein phosphorylated at Ser392 ~53 kDa p53 protein phosphorylated at Ser392. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser392

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Antigen NY-CO-13 antibody, BCC7 antibody, Cellular tumor antigen p53 antibody, FLJ92943 antibody, LFS1 antibody, Mutant tumor protein 53 antibody, p53 antibody, p53 tumor suppressor antibody, P53_HUMAN antibody, Phosphoprotein p53 antibody, Tp53 antibody, Transformation related protein 53 antibody, TRP53 antibody, Tumor protein 53 antibody, Tumor protein p53 antibody, Tumor suppressor p53 antibody

UniProt Details

UniProt (Human): P04637
UniProt (Immunogen Species): P04637

NCBI Gene ID

7157

Product Specific Protocols

Western Blot Protocol: Download

General References

Jiang, Y.H., Armstrong, D., Albrecht, U., Atkins, C.M., Noebels, J.L., Eichele, G., Sweatt, J.D. and Beaudet, A.L., 1998. Mutation of the Angelman ubiquitin ligase in mice causes increased cytoplasmic p53 and deficits of contextual learning and long-term potentiation. Neuron, 21(4), pp.799-811. PMID: 9808466
Kim, Y.Y., Park, B.J., Kim, D.J., Kim, W.H., Kim, S., Oh, K.S., Lim, J.Y., Kim, J., Park, C. and Park, S.I., 2004. Modification of serine 392 is a critical event in the regulation of p53 nuclear export and stability. FEBS letters, 572(1-3), pp.92-98. PMID: 15304330
Lane, D.P., 1992. Cancer. p53, guardian of the genome. Nature, 358, pp.15-16. PMID: 1614522
Pise-Masison, C.A., Radonovich, M., Sakaguchi, K., Appella, E. and Brady, J.N., 1998. Phosphorylation of p53: a novel pathway for p53 inactivation in human T-cell lymphotropic virus type 1-transformed cells. Journal of virology, 72(8), pp.6348-6355. PMID: 9658074
Saito, S.I., Yamaguchi, H., Higashimoto, Y., Chao, C., Xu, Y., Fornace, A.J., Appella, E. and Anderson, C.W., 2003. Phosphorylation site interdependence of human p53 post-translational modifications in response to stress. Journal of Biological Chemistry, 278(39), pp.37536-37544. PMID: 12860987
Sharpless, N.E. and DePinho, R.A., 2002. p53: good cop/bad cop. Cell, 110(1), pp.9-12. PMID: 12150992
Yin, Y., Tainsky, M.A., Bischoff, F.Z., Strong, L.C. and Wahl, G.M., 1992. Wild-type p53 restores cell cycle control and inhibits gene amplification in cells with mutant p53 alleles. Cell, 70(6), pp.937-948. PMID: 1525830

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Anti-p53 (Ser392) Antibody

Our Anti-p53 (Ser392) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and rat p53 (Ser392) and is antigen affinity purified from pooled serum. It is great for use in WB.