Anti-nSMase2 (C-terminal region) Antibody

Name: Anti-nSMase2 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: SP4061
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

4 Citations

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

SMPD3

Molecular Weight

Go to Full Product Details

Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).

Immunocytochemical labeling of nSMase2 in aldehyde-fixed and NP-40-permeabilized differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-nSMase2 (SP4061) antibody in the absence (Left) or presence (Right) of blocking peptide (SX4065). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594. The cells were counterstained with Sytox green to label nuclei.

Representative immunoblots showing expression of nSMase2 (cat. SP4061, 1:1000) at the indicated timepoints (0 to 48 h) following HIVRF infection of H9 cells. Image from publication CC-BY-4.0. PMID: 37406092

Western blot of adult mouse brain. The blots were probed with anti-nSMase2 (C-terminal region) rabbit polyclonal antibody at 1:2000 (lane 1), 1:1000 (lane 2), or 1:500 in the absence (lane 3) or presence of nSMase2 blocking peptide (SX4065) (lane 4).

Volume: 100 µL

Variant

Price:

$309

Quantity:

Bulk Order Anti-nSMase2 (C-terminal region) Antibody

Product Details

Target

nSMase2 (C-terminal region)

Target Description

Cellular stress leads to hydrolysis of sphingomyelin to generate lipid second messenger molecules including ceramide, sphingosine, and sphingosine-1-phosphate. A variety of sphingomyelinase activities have been described that differ in tissue and subcellular distribution, as well as pH and cation dependance. These enzymes generate ceramide for specific signaling pathways that lead to a wide range of cellular responses, such as apoptosis, cell cycle arrest, cell survival, and cell proliferation. Neutral sphingomyelinases (nSMases) are Mg2+-dependent neutral pH SMases, and the family includes nSMase1, nSMase2, and nSMase3. These nSMases differ in their tissue distribution and subcellular localization. nSMase2 (also known as SMPD3) is expressed primarily in brain, and co-localizes with Golgi markers in several cell lines. The activity and phosphorylation of nSMase2 is upregulated in response to oxidative stress. In addition, nSMase2 binds calcineurin, and this interaction inhibits nSMase2 activity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

SMPD3

Molecular Weight

Antigen

nSMase2 synthetic peptide corresponds to amino acids in the C-terminal region of human nSMase2. This sequence is highly conserved in rat and mouse nSMase2, and has low homology to other nSMase family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 78 kDa* band corresponding to nSMase2 on SDS-PAGE immunoblots of adult mouse brain and rat PC12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

SMPD3,neutral sphingomyelinase 2, nSMase sphingomyelin

UniProt Details

UniProt (Human): Q9NY59
UniProt (Immunogen Species): Q9NY59

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
27864331	1:100	Fu, P, et al. 2016. Role of Sphingosine Kinase 1 and S1P Transporter Spns2 in HGF-mediated Lamellipodia Formation in Lung Endothelium. The journal of biological chemistry, 27187-27203.

Western Blot: Human
PMID	Dilution	Publication
35178477	not listed	Dai, L, et al. 2022. Activation of SphK2 contributes to adipocyte-induced EOC cell proliferation. Open medicine, 229-238.
33525436	not listed	Bernacchioni, C, et al. 2021. Role of Sphingosine 1-Phosphate Signalling Axis in Muscle Atrophy Induced by TNFα in C2C12 Myotubes. International journal of molecular sciences, 1280.

Western Blot: Mouse
PMID	Dilution	Publication
24686171	not listed	Wallington-Beddoe, CT, et al. 2014. Sphingosine kinase 2 promotes acute lymphoblastic leukemia by enhancing MYC expression. Cancer research, 2803-2815.

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Anti-nSMase2 (C-terminal region) Antibody