Our Anti-NSE (Neuron specific enolase) primary antibody from PhosphoSolutions is rabbit polyclonal. It detects bovine, chicken, human, mouse, and rat NSE (Neuron specific enolase) and is neat serum. It is great for use in WB, IHC, ICC.
Immunolabeling of E20 rat mixed neuron and glial cultured cells labeled with anti-neuron specific enolase antibody (cat. 1520-NSE, red, 1:500) and anti-GFAP antibody ( cat. 621-GFAP , green, 1:5000). The blue is Hoechst stain of nuclear DNA. Anti-NSE labels protein expressed in neuronal cells and anti-GFAP labels protein expressed in intermediate filaments in astrocytic and other glial cells.
Anti-NSE (Neuron Specific Enolase) Antibody
NSE (Neuron specific enolase)
Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury (2).
ICC, IHC, WB
Recombinant human NSE expressed in and purified from E. coli.
Bovine, Chicken, Human, Mouse, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
WB Brain: 1:2000
Specific for endogenous levels of the ~47 kDa NSE protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
2 phospho D glycerate hydrolyase antibody, 2-phospho-D-glycerate hydro-lyase antibody, Eno 2 antibody, ENO2 antibody, ENOG antibody, ENOG_HUMAN antibody, Enolase 2 (gamma, neuronal) antibody, Enolase 2 antibody, Enolase 2 gamma neuronal antibody, Enolase2 antibody, Epididymis secretory protein Li 279 antibody, Gamma enolase antibody, Gamma-enolase antibody, HEL S 279 antibody, Neural enolase antibody, Neuron specific enolase antibody, Neuron specific gamma enolase antibody, Neuron-specific enolase antibody, neuronal enriched enolase antibody, Neurone specific enolase antibody, NSE antibody
MacAlesse SM, Dunbar B, Fotherqill JE, Hinks LJ, Day IN (1988) Complete amino acid sequence of neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). European Journal of Biochemistry. 178(2):413-17. PMID: 3208766
Begaz, T., Kyriacou, D. N., Segal, J. and Bazarian, J. J. Serum biochemical markers for post-concussion syndrome in patients with mild traumatic brain injury. Journal of Neurotrauma 23:1201-1210 (2006). PMID: 16928178
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