Our Anti-NMDA NR2B Subunit (Tyr1336) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Tyr1336) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Primary Antibody
Mouse, Rat
Human, Non-Human Primate
IHC, WB
Rabbit
Antigen Affinity Purified from Pooled Serum
GRIN2B
180 kDa


Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1336 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Product Specific References for Applications and Species
Immunocytochemistry: Rat | ||
PMID | Dilution | Publication |
32651756 | 1:100 | Akkuratov, E.E., et al. 2020. Ouabain Modulates the Functional Interaction Between Na, K-ATPase and NMDA Receptor. Molecular Neurobiology, pp.1-13. |
Immunohistochemistry: Mouse | ||
PMID | Dilution | Publication |
33096049 | not listed | Rodríguez-Matellán, A., et al. 2020. In Vivo Reprogramming Ameliorates Aging Features in Dentate Gyrus Cells and Improves Memory in Mice. Stem Cell Reports, 15(5), pp.1056-1066. |
31235881 | 1:400 | Pallas-Bazarra, N., et al. 2019. Tau is required for the function of extrasynaptic NMDA receptors. Scientific Reports, 9(1), p.9116. |
29101031 | 1:400 | Holmes, A., et al. 2018. A deficiency of the GluN2C subunit of the N-Methyl-d-Aspartate receptor is neuroprotective in a mouse model of ischemic stroke. Biochemical and Biophysical Research Communications. Jan 1;495(1):136-144. |
28993142 | 1:400 | Berrout, L., et al. 2018. Ghrelin upregulates the phosphorylation of the GluN2B subunit of the NMDA receptor by activating GHSR1a and Fyn in the rat hippocampus. Brain research, 1678, pp.20-26. |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
31235881 | 1:1000 | Pallas-Bazarra, N., et al. 2019. . Tau is required for the function of extrasynaptic NMDA receptors. Scientific Reports, 9(1), p.9116. |
30504838 | not listed | Minnella, A.M., et al. 2018. Excitotoxic superoxide production and neuronal death require both ionotropic and non-ionotropic NMDA receptor signaling. Scientific reports, 8(1), p.17522. |
27094400 | 1:1000 | Zamzow, D.R., et al. 2016. Higher levels of phosphorylated Y1472 on GluN2B subunits in the frontal cortex of aged mice are associated with good spatial reference memory, but not cognitive flexibility. AGE, 38(3), 1-17. |
25158771 | not listed | Knox, R., et al. 2014. NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia. Stroke, 45(10), pp.3040-3047. |
21464302 | 1:1000 | Ticklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655. |
17687025 | not listed | Chernova, T., et al. 2007. Neurite Degeneration Induced by Heme Deficiency Mediated via Inhibition of NMDA Receptor-Dependent Extracellular Signal-Regulated Kinase 1/2 Activation. J. Neurosci., 27: 8475 - 8485. |
Western Blot: Rat | ||
PMID | Dilution | Publication |
21464302 | 1:1000 | Ticklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655. |
Product Specific Protocols
- Western Blot Protocol: Download