Name: Anti-NMDA NR2B Subunit (Tyr1252) Antibody
Brand: PhosphoSolutions
SKU: p1516-1252
Price: 415.0 USD
Availability: InStock

Our Anti-NMDA NR2B Subunit (Tyr1252) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Tyr1252) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Zebrafish

Applications

IHC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified from Pooled Serum

Gene Name

GRIN2B

Molecular Weight

180 kDa

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Immunostaining of 14 DIV rat cortical neurons showing NR2B phosphorylated at Tyr1252 (red, 1:400) and PSD95 (green). Photo courtesy of Gang Liu.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit phosphorylated at Tyr1252 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Volume: 100 µL

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$415

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Bulk Order Anti-NMDA NR2B Subunit (Tyr1252) Antibody

Product Details

Target

NMDA NR2B Subunit (Tyr1252)

Target Description

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Phosphorylation of Tyr-1252 is thought to potentiate NMDA receptor-dependent influx of calcium (Takasu et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

GRIN2B

Molecular Weight

180 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 of the NR2B subunit of the rat NMDA receptor, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Human, Non-Human Primate, Zebrafish

Antibody Registry ID

AB_2492180

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:100-1:400

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~180 kDa NMDAR NR2B-subunit protein phosphorylated at Tyr1252 . Immunolabeling is completely eliminated by treatment with λ-phosphatase. May also show slight reactivity with Tyr1246 of NR2A.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Tyr1252

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

UniProt Details

UniProt (Human): Q13224
UniProt (Immunogen Species): Q00960

NCBI Gene ID

24410

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Western Blot: Mouse | Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
32651756	1:100	Akkuratov, E.E., et al. 2020. Ouabain Modulates the Functional Interaction Between Na, K-ATPase and NMDA Receptor. Molecular Neurobiology, pp.1-13.

Western Blot: Mouse
PMID	Dilution	Publication
32946951	1:1000	Diab, A., et al. 2020. NCK1 regulates amygdala activity to control context-dependent stress responses and anxiety in male mice. Neuroscience, 448, pp 107-125.
25158771	not listed	Knox, R., et al. 2014. NR2B phosphorylation at tyrosine 1472 contributes to brain injury in a rodent model of neonatal hypoxia-ischemia. Stroke, 45(10), pp.3040-3047.
21464302	1:1000	HIcklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655.

Western Blot: Rat
PMID	Dilution	Publication
21464302	1:1000	HIcklin, T.R., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. PNAS, Apr 2011; 108: 6650 - 6655.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-NMDA NR2B Subunit (Tyr1252) Antibody

Our Anti-NMDA NR2B Subunit (Tyr1252) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat NMDA NR2B Subunit (Tyr1252) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.