Name: Anti-NMDA NR2B Subunit Antibody
Brand: PhosphoSolutions
SKU: 1498-NR2B
Price: 360.0 USD
Availability: InStock

Our Anti-NMDA NR2B Subunit rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2B Subunit and is neat pooled serum. It is great for use in WB, ICC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

WB

Host Species

Rabbit

Format

Neat Pooled Serum

Gene Name

GRIN2B

Molecular Weight

180 kDa

Go to Full Product Details

Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA receptor.

Immunofluorescence of healthy iPSC-derived neurons labeled with Anti-NMDA Receptor, NR2B subunit (cat. 1498-NR2B, green, 1:100) and Anti-TuJ1 (red). The blue is Hoechst staining of nuclear DNA. Image kindly provided by Dr. Allison Ebert, Medical College of Wisconsin.

Volume: 50 µL

Variant

Price:

$360

Quantity:

Bulk Order Anti-NMDA NR2B Subunit Antibody

Product Details

Target

NMDA NR2B Subunit

Target Description

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Format

Neat Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

WB

Host Species

Rabbit

Gene Name

GRIN2B

Molecular Weight

180 kDa

Antigen

Fusion protein from the C-terminal region of the NR2B subunit of the rat NMDA receptor.

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492177

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Unpurified pooled neat serum.

Buffer

Neat serum

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~180 kDa NR2B subunit of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the fusion protein used to generate the antibody. No reactivity towards the NR2A and NR2C subunits.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

EPND antibody, FESD antibody, GluN2A antibody, Glutamate [NMDA] receptor subunit epsilon-1 antibody, Glutamate receptor antibody, Glutamate receptor ionotropic N methyl D aspartate 2A antibody, GRIN 2A antibody, GRIN2A antibody, hNR2A antibody, LKS antibody, N methyl D aspartate receptor channel subunit epsilon 1 antibody, N Methyl D Aspartate Receptor Subtype 2A antibody, N methyl D aspartate receptor subunit 2A antibody, N-methyl D-aspartate receptor subtype 2A antibody, NMDA receptor subtype 2A antibody, NMDAR 2A antibody, NMDAR2A antibody, NMDE1_HUMAN antibody, NR2A antibody, OTTHUMP00000160135 antibody, OTTHUMP00000174531 antibody

UniProt Details

UniProt (Human): Q13224
UniProt (Immunogen Species): Q00960

NCBI Gene ID

24410

Product Specific References for Applications and Species

Immunocytochemistry: Human
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
29129681	1:100	Schwab, A.J., et al. 2017. Decreased Sirtuin Deacetylase Activity in LRRK2 G2019S iPSC-Derived Dopaminergic Neurons. Stem Cell Reports, Vol 9, 1-14, Dec 12.

Western Blot: Human
PMID	Dilution	Publication
29129681	not listed	Schwab, A.J., et al. 2017. Decreased Sirtuin Deacetylase Activity in LRRK2 G2019S iPSC-Derived Dopaminergic Neurons. Stem Cell Reports, Vol 9, 1-14, Dec 12.

Western Blot: Mouse
PMID	Dilution	Publication
25971971	1:1000	Priya, A., et al. 2013. Specificity protein 4 functionally regulates the transcription of NMDA receptor subunits GluN1, GluN2A, and GluN2B. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 1833(12), 2745-2756.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-NMDA NR2B Subunit Antibody

Our Anti-NMDA NR2B Subunit rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2B Subunit and is neat pooled serum. It is great for use in WB, ICC.