Our Anti-NMDA NR1 Subunit primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat NMDA NR1 Subunit and is culture supernatant. It is great for use in WB, IHC, ICC.
Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~120 kDa NR1 subunit of the NMDA receptor.
The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to Nmethyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1-subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.
Culture supernatant
Monoclonal
R1JHL
IgG
ICC, IHC, WB
Mouse
GRIN1
120 kDa
Fusion protein containing amino acids 1-564 of the NR1 subunit of rat NMDA receptor.
Human, Mouse, Rat
AB_2492165
Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Tanaka, K., et al. 2021. Evaluation of the concordance between GluN1-GluN2 heteromer live-cell-based assay and GluN1 monomer biochip kit assay on anti-NMDAR autoantibody detection. Journal of Immunological Methods, 499, p.113150.
Quillinan, N., et al. 2015. Region-specific role for GluN2B-containing NMDA receptors in injury to Purkinje cells and CA1 neurons following global cerebral ischemia. Neuroscience, 284, 555-565.
Zhang, H., Mu, L., Dandan, W., Dongdong, X. et al. 2018. Uncovering a critical period of synaptic imbalance during postnatal development of the rat visual cortex: role of brain‐derived neurotrophic factor. The Journal of Physiology. Sep;596(18):4511-4536.
Zhang, G., et al. 2008. Isoflurane-Induced Caspase-3 Activation Is Dependent on Cytosolic Calcium and Can Be Attenuated by Memantine. J. Neurosci., 4551 - 4560.
Hicklin, T., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. Proc Natl Acad Sci USA. 108(16):6650-5.
Zhang, H., Mu, L., Dandan, W., Dongdong, X. et al. 2018. Uncovering a critical period of synaptic imbalance during postnatal development of the rat visual cortex: role of brain‐derived neurotrophic factor. The Journal of Physiology. Sep;596(18):4511-4536.
Hicklin, T., et al. 2011. Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase. Proc Natl Acad Sci USA. 108(16):6650-5.
Davies, K., et al. 2008. Long Term Synaptic Depression That Is Associated with GluR1 Dephosphorylation but Not -Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid (AMPA) Receptor Internalization. J. Biol. Chem., 283: 33138 - 33146.
