Anti-NLRC4 (Ser-533), Phosphospecific Antibody

Name: Anti-NLRC4 (Ser-533), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: NP5411
Price: 329.0 USD
Availability: InStock

4 Citations

Our NLRC4 (Ser-533) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NLRC4 (Ser-533) and is antigen affinity purified. It is great for use in WB.

Western blot image of mouse recombinant NLRC4 (Ser-533) phosphorylated peptide (lanes 1 & 4) and dephosphorylated peptide (lanes 2 & 5), as well as human PMA-differentiated THP1 cells (lanes 3 & 6). The blots were probed with rabbit polyclonals anti-NLRC4 (a.a. 525-538) (lanes 1-3) and anti-NLRC4 (Ser-533) phospho-specific (lanes 4-6).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: NP5411

Product Details

Target

NLRC4 (Ser-533)

Target Description

The nucleotide-binding oligomerization domain (NOD)-like receptor (NLR) family is a diverse family of cytoplasmic innate immune receptors that are involved in recognition of pathogen-associated molecular patterns. NLRs are important for pathogen sensing, transcriptional activation of proinflammatory cytokines and activation of inflammatory caspases. NLRC4 (IPAF, CARD12) forms the inflammasome that responds to bacterial flagellin. This inflammasome is activated by NLRC4 oligomerization, NAIP protein binding, and activation of caspase-1 leading to pyroptosis. NLRC4 is phosphorylated on Ser-533 by PKCδ following infection of macrophages with S. typhimurium. Mutant forms of NLRC4 demonstrate that an unphosphorylatable form (S533A) does not activate caspase-1 and pyroptosis in response to S. typhimurium, while a phosphomimetic NLRC4 (S533D) mutant causes rapid macrophage pyroptosis without infection. Thus, PKCδ phosphorylation of NLRC4 (S533) may be a critical event in inflammasome activation and host innate immunity.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

NLRC4

Molecular Weight

110

Antigen

Phospho-NLRC4 (Ser-533) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding serine 533 in mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody was cross-adsorbed to unphosphorylated NLRC4 (Ser-533) peptide before affinity purification using phospho-NLRC4 (Ser-533) peptide. This antibody antibody detects a 110 kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) peptide, but does not detect this peptide after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-533

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CARD12, CLAN1, IPAF, NLR family CARD domain-containing protein 4, NOD-like receptor 4

UniProt Details

UniProt (Human): Q9NPP4
UniProt (Immunogen Species): Q3UP24

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: mouse
PMID	Dilution	Publication
35277504	1:50	Mohamed, MF, et al. 2022. CrkII/Abl phosphorylation cascade is critical for NLRC4 inflammasome activity and is blocked by Pseudomonas aeruginosa ExoT. Nature communications, 1295.
33893171	not listed	Ni, J, et al. 2021. Ubc13 Promotes K63-Linked Polyubiquitination of NLRP3 to Activate Inflammasome. Journal of immunology (Baltimore, Md. : 1950), 2376-2385.
30279514	not listed	Paudel, S, et al. 2019. NLRC4 suppresses IL-17A-mediated neutrophil-dependent host defense through upregulation of IL-18 and induction of necroptosis during Gram-positive pneumonia. Mucosal immunology, 247-257.
28821668	not listed	Liu, W, et al. 2017. LRRK2 promotes the activation of NLRC4 inflammasome during Salmonella Typhimurium infection. The Journal of experimental medicine, 3051-3066.

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Anti-NLRC4 (Ser-533), Phosphospecific Antibody

SKU: NP5411

Product Details

Product Specific References for Applications and Species

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