Anti-NFM (Neurofilament Medium Chain) Antibody

NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.


Volume: 200 µL
Sale price$285

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
294044021:500Hines, T.J., et al. 2018. An Essential Postdevelopmental Role for Lis1 in Mice. eNeuro, .
Immunocytochemistry: Rat
PMID Dilution Publication
339423141:1000Molina-Holgado, E., et al. 2021. Cannabinoid Receptor 1 associates to different molecular complexes during GABAergic neuron maturation. Journal of Neurochemistry, 640-656.
300380331:500Lee, S.J., et al. 2018. hnRNPs interacting with mRNA localization motifs define axonal RNA regulons. Molecular & Cellular Proteomics, 2091-2106.
259199461:500Kim, H.H., et al. 2015. Identification of precursor micro RNA s within distal axons of sensory neuron. Journal of Neurochemistry, 193-199.
Immunohistochemistry: Human
PMID Dilution Publication
229117631:1000Bin, J.M., et al. 2012. Oligodendrocyte precursor cell transplantation into organotypic cerebellar shiverer slices: a model to study myelination and myelin maintenance. PLoS One, e41237.
Immunohistochemistry: Mouse
PMID Dilution Publication
288801491:1,000Figlia, G., et al. 2017. Dual function of the PI3K-Akt-mTORC1 axis in myelination of the peripheral nervous system. eLife, e29241.
Immunohistochemistry: Rat
PMID Dilution Publication
326570691:500Wang, S., et al. 2020. Identification of Medium-Length Antineurofilament Autoantibodies in Patients with Anti-N-Methyl-D-Aspartate Receptor Encephalitis. Journal of Clinical Neurology, 470-479.
303642181:500Arevalo-Martin, A., et al. 2018. Elevated Autoantibodies in Subacute Human Spinal Cord Injury Are Naturally Occurring Antibodies. Frontiers in Immunology, 2365.
294540141:100Gluck, M.J., et al. 2018. Detecting structural and inflammatory response after in vivo stretch injury in the rat median nerve via second harmonic generation. Journal of Neuroscience Methods, 68-80.

Related Products

Recently Viewed