Anti-Myosin IIA Heavy Chain (Ser-1943), Phosphospecific Antibody

Name: Anti-Myosin IIA Heavy Chain (Ser-1943), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: MP3831
Price: 309.0 USD
Availability: InStock

from PhosphoSolutions

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Format

Antigen Affinity Purified

Gene Name

MYH9

Molecular Weight

200

Go to Full Product Details

Immunocytochemical labeling of myosin IIA heavy chain phosphorylation relative to F-actin in chick fibroblasts. The cells were labeled with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943) antibody (MP3831), then detected using appropriate secondary antibody (Bottom, Red). This labeling is compared to F-actin staining (Bottom, Green) and to secondary only (Top). (Image provided by Dr. Gianluca Gallo at Drexel University).

Western blot image of human A431 cells. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with rabbit polyclonal Myosin IIA Heavy Chain (Ser-1943), phospho-specific antibody (lanes 1 & 2) or rabbit polyclonal Myosin IIA Heavy Chain (a.a. 1936-1950) antibody (lanes 3 & 4).

Volume: 100 µL

Variant

Price:

$309

Quantity:

Bulk Order Anti-Myosin IIA Heavy Chain (Ser-1943), Phosphospecific Antibody

Product Details

Target

Myosin IIA Heavy Chain (Ser-1943), phospho-specific

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MYH9

Molecular Weight

200

Antigen

Phospho-NMHC-IIA (Ser-1943) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-1943 in human myosin IIA heavy chain. This peptide sequence is highly conserved in rat and mouse NMHC-IIA, but is not found in NMHC-IIB and is not well conserved in NMHC-IIC.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

This antibody was cross-adsorbed to unphosphorylated NMHC-IIA (Ser-1943) peptide before affinity purification using phospho-NMHC-IIA (Ser-1943) peptide (without carrier). The antibody detects a 200 kDa* protein corresponding to NMHC-IIA on SDS-PAGE immunoblots of human A431, Jurkat, mouse C2C12, and rat PC12 cells. This reactivity is removed after treatment with lambda phosphatase. The antibody also detects NMHC-IIA (Ser-1943) phosphorylation in chick Fibroblasts.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

S1943

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

NMHC-IIA, MYH9, myosin heavy chain

UniProt Details

UniProt (Human): P35579
UniProt (Immunogen Species): P35579

Product Specific Protocols

Western Blot Protocol: Download

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Anti-Myosin IIA Heavy Chain (Ser-1943), Phosphospecific Antibody