Our Anti-Myelin Basic Protein (MBP) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Myelin Basic Protein (MBP) and is total IgY fraction. It is great for use in WB, IHC, ICC.
Immunofluorescence of a section of rat cerebellum showing specific labeling of myelin basic protein (cat. 1120-MBP, 1:5000, green) in the oligodendrocytes and myelin sheathes around axons. The section is colabeled with Anti-NFL (red) labeling dendrites and axons of neuronal cells. The blue stains nuclear DNA.
Anti-Myelin Basic Protein (MBP) Antibody
Bulk Order Anti-Myelin Basic Protein (MBP) Antibody
Myelin Basic Protein (MBP)
Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago (Eylar et al., 1971). The protein is made by oligodendrocytes in the central nervous system, so antibodies to MBP are good markers of this cell type. However, transcripts from the same gene are also expressed in certain hematopoetic lineage cells (Marty et al., 2002). In the central nervous system there are there different forms of the protein made by alternate transcription from a single gene, which have molecular weights of 21.5, 18.5, and 17.2 kDa. Since the two lower molecular weight forms are very close in molecular size, MBP antibodies typically show two bands on Western blots, one at about 22 kDa and another at about 18 kDa.
Total IgY fraction
ICC, IHC, WB
Three peptide sequences conserved in higher vertebrate MBP proteins
Bovine, Human, Mouse, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Gatto, R.G., Ye, A.Q., et al. 2019. Detection of axonal degeneration in a mouse model of Huntington's disease: comparison between diffusion tensor imaging and anomalous diffusion metrics. MAGMA, Feb 15.