Our Anti-Metabotropic Glutamate Receptor 5/1a primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat Metabotropic Glutamate Receptor 5/1a and is antigen affinity purified. It is great for use in WB, IHC.
Western blot of 10 µg of HEK 293 cells expressing: 1) mGluR5 and 2) mGluR1a showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimers of both mGluR1a (2) and mGluR5 (1).
The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).
Antigen Affinity Purified
Polyclonal
IgG
IHC, WB
Rabbit
GRM5
125/250 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat mGluR5 and rat mGluR1a, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Human
AB_2492152
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum using a column to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
IHC: 1:500
Unconjugated
Specific for endogenous levels of the ~125 kDa monomer and the ~250 kDa dimers of mGluR5 and mGluR1. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
