Name: Anti-MeCP2 (Ser80) Antibody
Brand: PhosphoSolutions
SKU: p1205-80
Price: 360.0 USD
Availability: InStock

Our Anti-MeCP2 (Ser80) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat MeCP2 (Ser80) and is serum. It is great for use in WB, IHC.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

IHC, WB

Host Species

Rabbit

Format

Serum

Gene Name

MECP2

Molecular Weight

75 kDa

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Western blot of neonatal rat brain showing specific immunolabeling of the ~75 kDa MeCP2 protein phosphorylated at Ser80.

Volume: 100 µL

Variant

Price:

$360

Quantity:

Bulk Order Anti-MeCP2 (Ser80) Antibody

Product Details

Target

MeCP2 (Ser80)

Target Description

MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser-80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors. (Tao et al., 2009)

Format

Serum

Clonality

Polyclonal

Isotype

IgG

Applications

IHC, WB

Host Species

Rabbit

Gene Name

MECP2

Molecular Weight

75 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 of human MeCP2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492144

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Serum

Buffer

Serum

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~75 kDa MeCP2 protein phosphorylated at Ser80 in Western blots of human, rat and mouse brain extracts. The antibody has also been used successfully for immunohistochemistry on mouse brain sections.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser80

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

AUTSX 3 antibody, AUTSX3 antibody, DKFZp686A24160 antibody, Mbd 5 antibody, Mbd5 antibody, MECP 2 antibody, MeCP 2 protein antibody, MeCP-2 protein antibody, Mecp2 antibody, MECP2_HUMAN antibody, Methyl CpG binding protein 2 (Rett syndrome) antibody, Methyl CpG binding protein 2 antibody, Methyl-CpG-binding protein 2 antibody, MRX 16 antibody, MRX 79 antibody, MRX16 antibody, MRX79 antibody, MRXS 13 antibody, MRXS13 antibody, MRXSL antibody, PPMX antibody, RS antibody, RTS antibody, RTT antibody, WBP 10 antibody, WBP10 antibody

UniProt Details

UniProt (Human): P51608
UniProt (Immunogen Species): P51608

NCBI Gene ID

4204

Product Specific References for Applications and Species

Immunohistochemistry: Human | Mouse
Western Blot: Human | Mouse

Immunohistochemistry: Human
PMID	Dilution	Publication
28367114	1:250	Maphis, N.M., et al. 2017. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28367114	1:1000	Maphis, N.M., et al. 2017. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.
19225110	not listed	Tao, J., et al. 2009. Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function. Proc Natl Acad Sci U S A 106(12):4882-7.

Western Blot: Human
PMID	Dilution	Publication
28367114	1:250	Maphis, N.M., et al. 2017. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.

Western Blot: Mouse
PMID	Dilution	Publication
28367114	1:1000	Maphis, N.M., et al. 2017. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.
19225110	not listed	Tao, J., et al. 2009. Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function. Proc Natl Acad Sci U S A 106(12):4882-7.

Product Specific Protocols

Western Blot Protocol: Download

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Anti-MeCP2 (Ser80) Antibody

Our Anti-MeCP2 (Ser80) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat MeCP2 (Ser80) and is serum. It is great for use in WB, IHC.