Our Anti-MeCP2 (Ser80) phosphospecific primary antibody from PhosphoSolutions is rabbit polyclonal. It detects human, mouse, and rat MeCP2 (Ser80) and is serum. It is great for use in WB, IHC.
Western blot of neonatal rat brain showing specific immunolabeling of the ~75 kDa MeCP2 protein phosphorylated at Ser80.
MECP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MECP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Defects in MECP2 are the cause of Rett syndrome (RTT). RTT is an X-linked dominant disease, it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Recent studies have reported a new phosphorylation site at Ser-80. Phosphorylation and dephosphorylation of this site may be involved in modulating the dynamic function of MECP2 in neurons transiting between resting and active states within neural circuits that underlie behaviors. (Tao et al., 2009)
Serum
Polyclonal
IgG
IHC, WB
Rabbit
MECP2
75 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser80 of human MeCP2, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse, Rat
AB_2492144
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Serum
Serum
WB: 1:1000
WB Brain: 1:1000
IHC: 1:100-1:250
Unconjugated
Specific for endogenous levels of the ~75 kDa MeCP2 protein phosphorylated at Ser80 in Western blots of human, rat and mouse brain extracts. The antibody has also been used successfully for immunohistochemistry on mouse brain sections.
Phosphorylated
Ser80
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Maphis, N.M., et al. 2017. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.
Maphis, N.M., et al. 2017. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.
Tao, J., et al. 2009. Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function. Proc Natl Acad Sci U S A 106(12):4882-7.
Maphis, N.M., et al. 2017. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.
Maphis, N.M., et al. 2017. Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology. Frontiers in Molecular Neuroscience, 10:69.
Tao, J., et al. 2009. Phosphorylation of MeCP2 at Serine 80 regulates its chromatin association and neurological function. Proc Natl Acad Sci U S A 106(12):4882-7.
