Our Anti-MeCP2 (Ser421) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat MeCP2 (Ser421) and is antigen affinity purified from pooled serum. It is great for use in WB.
Primary Antibody
Mouse, Rat
Human, Non-Human Primate
WB
Rabbit
Antigen Affinity Purified from Pooled Serum
MECP2
55 kDa

Western blot of mouse whole brain lysate showing specific immunolabeling of the ~55 kDa truncated MeCP2 protein phosphorylated at Ser421 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Product Specific References for Applications and Species
- Western Blot: Mouse
Western Blot: Mouse | ||
PMID | Dilution | Publication |
32138755 | 1:1000 | Buck, J.M., et al. 2020. Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice. Epigenetics and Chromatin, 13(1), pp.1-15. |
Product Specific Protocols
- Western Blot Protocol: Download