Our Anti-MeCP2 Antibody primary antibody from PhosphoSolutions is chicken polyclonal. It detects human, mouse, non-human primate, and rat MeCP2 and is total IgY fraction. It is great for use in WB, IHC.
Immunostaining of a section of rat olfactory bulb showing specific labeling of MeCP2 in neuronal nuclei (Cat. No. 1207-MECP2, red, 1:2000) and alpha synuclein (green). The blue is DAPI staining of nuclear DNA.
MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Total IgY fraction
Full length human recombinant MeCP2
Human, Mouse, Non-Human Primate, Rat
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 µL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles.