Western blot analysis of mDia1 expression in human Jurkat cells (lanes 1-4). The blots were probed with anti-mDia1 (a.a. 66-77: DP4471) in the presence (lane 1) or absence of blocking peptide (DX4475) using dilutions of 1:250 (lane 2), 1:1000 (lane 3), and 1:4000 (lane 4).
Anti-mDia1 (N-terminal region) Antibody
SKU: DP4471
Bulk Order Anti-mDia1 (N-terminal region) Antibody
Product Details
mDia1 (N-terminal region)
Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia1 protein is activated by Rho and leads to ROCK-dependent stress fiber formation. Rho-activated mDia1 regulates serum response factor-dependent transcription. In cancers, mDia1 has been implicated in ras-mediated transformation, metastasis, and invasion. Thus, mDia1 is a Rho-activated formin with both cytoskeletal- and transcription-reguatling activities.
Antigen Affinity Purified
Polyclonal
IgG
WB
Rabbit
DIAPH1
140
mDia1 synthetic peptide corresponding to amino acids from the N-terminal region of human mDia1 (Diap1). This peptide sequence is highly conserved in rat and mouse mDia1, and has low homology to other formins.
Human, Mouse, Rat
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
WB: 1:1000
Unconjugated
The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of mDia1 on SDS-PAGE immunoblots of human K562, human Jurkat, and mouse C2C12 cells.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
United States
After date of receipt, stable for at least 1 year at -20°C.
