Anti-MAP3K12 Antibody (N377/20)

Name: Anti-MAP3K12 Antibody
Brand: NeuroMab
SKU: 75-355
Price: 374.0 USD
Availability: InStock

Our Anti-MAP3K12 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N377/20. It is KO validated, detects mouse and rat MAP3K12, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

$Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and gene- trap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis).$

Adult rat brain immunohistochemistry.

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

KO Validated

SKU: 75-355

Product Details

Target

Mitogen-activated protein kinase kinase kinase 12 (EC 2.7.11.25) (Dual leucine zipper bearing kinase) (DLK) (Leucine-zipper protein kinase) (ZPK) (MAPK-upstream kinase) (MUK) (Mixed lineage kinase)

Target Description

Mitogen-activated protein kinase kinase kinase 12, or MAP3K12 is a member of the serine/threonine protein kinase family. MAP3K12 is expressed many tissues including kidney, lung, heart, testis, gastrointestinal tract, stomach, liver and pancreas. In brain, MAP3K12 is mainly expressed in neurons at synaptic terminals. MAP3K12 is thought to initiate signalling cascades which effect the developing mammalian nervous system, including processes such as neuronal migration and axon growth.

Format

Purified

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N377/20

Isotype

IgG2a

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Map3k12 Zpk

Molecular Weight

120 kDa

Antigen

Fusion protein amino acids 727-888 (C-terminus) of mouse MAP3K12 (also known as Mitogen activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mixed lineage kinase, DLK, ZPK and MUK, accession number Q60700); Rat: 97% identity (158/162 amino acids identical); Human: 86% identity (143/166 amino acids identical); Less than 40% identity with MAP3K13

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2315888

Storage

Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Buffer

10mM Tris, 50mM Sodium Chloride, 0.065 % Sodium Azide pH 7.4

Conjugate

Unconjugated

Specificity

Does not cross-react with MAP3K13

Quality Control

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from opening

Synonyms

Mitogen-activated protein kinase kinase kinase 12 (EC 2.7.11.25) (Dual leucine zipper bearing kinase) (DLK) (Leucine-zipper protein kinase) (ZPK) (MAPK-upstream kinase) (MUK) (Mixed lineage kinase)

UniProt Details

UniProt (Human): Q12852
UniProt (Immunogen Species): Q60700

Product Specific References for Applications and Species

Western Blot: Mouse
Additional Publications: Unspecified

Western Blot: Mouse
PMID	Dilution	Publication
35101451	not listed	Lee B, et al. 2022. FK506-binding protein-like and FK506-binding protein 8 regulate dual leucine zipper kinase degradation and neuronal responses to axon injury. The Journal of biological chemistry, 101647.

Additional Publications: Unspecified
PMID	Publication
32659913	Goodwani S, et al. 2020. Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions. International Journal of Molecular Sciences, 4849.

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Anti-MAP3K12 Antibody (N377/20)

SKU: 75-355

Product Details

Product Specific References for Applications and Species

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